摘要
为了提高萝卜硫素的纯度,研究了大孔树脂分离纯化萝卜硫素工艺。通过静态吸附和静态洗脱实验,从8种不同性质的树脂中筛选出适合分离萝卜硫素SP850树脂,并对其动态吸附和动态洗脱条件进行了研究。结果表明:SP850树脂处理约20 BV(1BV=10mL)萝卜硫素溶液时才开始泄漏,并且动态吸附曲线可以用Bo-hart-Adams数学模型描述。SP850树脂纯化萝卜硫素的适宜的工艺条件为:上柱液体积为20 BV,上柱流速为5BV/h,洗脱液乙醇的体积分数为50%,洗脱液体积为6BV,洗脱速度为3 BV/h,在此条件下,吸附量为32.83 mg/mL树脂,洗脱率高达91.84%,萝卜硫素产品纯度可达88.7%。
In order to improve the purity of sulforaphen, the separation and purification processes for sultoraphen by macroporous resin adsorbent was studied. SP850 was selected as the best by static adsorption and desorption experiments. The conditions of dynamic adsorption and dynamic elution were also investigated. The biggest volume of SP850 resin adsorption was 20 BV sulforaphen solution and its adsorption curve fit Bohart-Adams model. The optimum technology parameters were ： sulforaphen solution volume 20BV, flow rate 5BV/h, 50% ethanol for elution, elution volume 20BV, elution velocity 3BV/h. Under the above conditions, the adsorption capacity was 32.83mg/mL resin. The elution field was as high as 91.84% ,and the purity of sulforaphen product reached 88.7%. The research results provided a reference for sulforaphen industrial purification.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第7期197-200,共4页
Food and Fermentation Industries
基金
上海市大学生创新资助项目(cs0904002)
上海工程技术大学基金资助项目(A-2500-10-01006)
关键词
萝卜硫素
大孔吸附树脂
分离
提纯
西兰花种子
sulforaphen, macroporous resin adsorbent, separation, purification, broccoli seed
