摘要
目的研究肺腺癌患者外周血中Th17细胞比例变化及其意义。方法收集28例肺腺癌患者与42例健康志愿者外周血标本。采用流式细胞术检测外周血Th17细胞比例,实时定量PCR技术检测外周血单个核细胞RORct、Foxp3mRNA表达水平,酶联免疫吸附法(ELISA)检测血清中IL-17A、TGF-β、IL-10的水平,所有标本同时进行肿瘤标志物系列检测。结果与健康对照组相比,肺腺癌患者外周血Th17细胞比例较健康对照组显著升高[1.19(0.89~1.88)%vs2.23(1.28~3.24)%,P=0.001],其特征性转录调控因子RORct及细胞因子IL-17A也明显升高(P<0.001,P=0.001)。外周血T调节细胞特征性转录调控因子Foxp3mRNA水平也显著升高(P=0.019),同时伴TGF-β及IL-10水平升高(P=0.014,P=0.003)。血清IL-17A与TGF-β之间呈显著正相关(P=0.006,r=0.497),外周血Th17细胞比例与CEA水平呈显著负相关(P=0.024,r=-0.502)。结论肺腺癌患者外周血Th17细胞比例及IL-17A水平显著高于健康对照组。Th17比例上调可能是肺腺癌患者免疫抑制的继发性改变。
Objective To investigate the frequency of Thl7 cells and IL-17A expression in patients with lung adenocar- cinoma, and explore its clinical significance. Methods Frequencies of Thl7 cells were detected in 28 patients with lung adenocarcinoma and 42 healthy volunteers by flow cytometry. Real time-PCR was used to detect levels of RORct and Foxp3 mRNA in peripheral blood mononuclear cells（ PBMC）. Enzyme-linked immunosorbent assay （ELISA） was used to detect serum concentrations of IL-17A, TGF-13 and IL-10. And serum levels of tumor markers were detected in the Department of Clinical Laboratory, Qilu hospital of Shandong University. Results The frequency of Thl7 cells in patients with lung adenocarcinoma was significantly higher than that in healthy vohmteers^2.23 （1.28-3.24） % vs 1.19 （0.89-1.88） % ,P = 0. 001 ~, and expressions of RORct mRNA and IL-17A were also up-regulated（ P 〈 0. 001, P = 0. 001 ）. Expression of Foxp3 mRNA was up-regulated in patients with lung adenocarcinoma（ P = 0.019）, accompanied by elevated serum concentrations of IL-10 and TGF-[3（ P = 0. 014, P = 0.003 ）. According to correlation analysis, the level of 1L-17A was positively correlated with that of TGF-[3 （P = 0. 006, r = 0. 497 ）, while the frequency of Thl7 cells was negatively correlated with serum concentration of carcinoembryonic antigen （CEA） （ P = 0. 024, r = - 0. 502 ）. Conclusion In the peripheral blood from patients with lung adenocarcinoma, the proportion of Thl7 cells and serum concentration of IL-17A are higher than those in healthy volunteers, which may be a significant compensation for sup- pressed anti-tumor immunity.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第8期108-112,共5页
Journal of Shandong University:Health Sciences
