摘要
目的:研究羟基喜树碱(HCPT)对体外培养的人眼Tenon囊成纤维细胞(Human Tenon's capsule fibroblasts,HTFs)增殖、移行的影响。方法:取正常供体新鲜的Tenon囊组织,采用组织块培养法,进行成纤维细胞的体外培养,并用光镜、免疫荧光法观察鉴定;MTT法、划痕法检测不同浓度的HCPT(0、0.031、0.062、0.125、0.25、0.5、1、2、4mg/l)对HTFs增殖、移行的影响,并与MMC对比。结果:HTFs体外生长良好,经光镜和免疫荧光法观察鉴定为成纤维细胞;与空白对照组比较,HCPT(0.031-4mg/l)、MMC(0.0031-0.4mg/l)均能有效抑制HTFs的增殖,且呈一定的剂量、时间依赖性,HCPT作用24h、48h、72h的IC50分别为2.24mg/l、0.76mg/l、0.39mg/l,MMC作用24h、48h、72h的IC50分别为0.34mg/l、0.24mg/l、0.07mg/l;与空白对照组比较,HCPT(0.031-4mg/1)、MMC(0.0031-0.4mg/l)均能抑制HTFs迁移,呈剂量依赖性,而与时间无显著相关。结论:HCPT、MMC均能有效抑制HTFs的增殖和移行,其效应MMC约为HCPT的10倍。
Objective： To investigate the effect of 10-hydroxycamptothecin on the proliferation, migration of human Tenon＇s capsule fibroblasts. Methods： Fresh donor of normal Tenon capsule, cultured fibroblasts in vitro using tissue culture were obtained. HTFs was detected by using light microscopy, immunofluorescence; The inhibitory effect of HCPT at different concentrations on HTFs （0,0.031, 0.062, 0.125, 0.25, 0.5, 1, 2, 4mg/1） was measured by methylthiazlyltetrazolium （MTT） assay; Scratch assays were used to evaluate the migration of HTFs. Results： HTFs were cultured successfully in vitro. The cultured cells were confirmed to be fibroblasts with light microscopy and immunofluorescenc; Compared with that in the control group, proliferation activity of HTFs was inhibited with dose and time dependent of HCPT （0.031-4mg / 1）, MMC （0.0031-0.4mg / 1）, IC50 of HCPT on HTFs for 24h, 48h, 72h was 2.24mg/1, 0.76mg/1, 0.39mg/1, IC50 of MMC on HTFs was 0.34mg/1, 0.24mg/1, 0.07mg/1; Compared with that the control group, HCPT （0.031-4mg / 1）, MMC （0.0031-0.4mg / 1） could inhibit HTFs migration which was in a dose dependent manner, but it has no significant correlation with the time; Conclusion： HCPT and MMC can inhibit the proliferation and migrationof HTFs, the effect of MMC is about 10 times that of the HCPT.
出处
《现代生物医学进展》
CAS
2011年第16期3067-3070,共4页
Progress in Modern Biomedicine
基金
江苏省教育厅科学基础研究资助项目(07KJB310070)
