摘要
目的研究分离自尿标本的大肠埃希菌(E.coli)Ⅰ、Ⅱ类整合子分布情况,并进行基因分型。方法以WHONET5.4软件对100株分离自尿标本的E.coli进行耐药性分析,聚合酶链反应(PCR)检测Ⅰ、Ⅱ类整合子,以肠杆菌科重复序列-聚合酶链反应(ERIC-PCR)进行基因分型。结果 100株E.coli对氨苄西林、氨曲南、头孢他啶、环丙沙星、头孢噻肟、头孢唑林、庆大霉素、左氧氟沙星、哌拉西林、复方新诺明、四环素耐药率较高,对碳青霉烯类抗菌剂耐药率为0.0%。Ⅰ类整合子检出率为66%,未检出Ⅱ类整合子。100株E.coli分为79种基因型。结论Ⅰ类整合子广泛存在于E.coli,并与其耐药性相关;ERIC-PCR可用于临床分离E.coli菌株的基因分型。
Objective To investigate the distribution of class Ⅰand Ⅱ integron and analyze the genotype of Escherichia coli（E.coli）strains isolated from urine samples.Methods Drug resistance was analyzed by WHONET5.4 software.entrobacter repetive intergenic consensus-polymerase chain reaction（ERIC-PCR）was performed to analyze the genotype of isolated strains.Genes,coding class Ⅰand Ⅱ integron,were detected by PCR.Results The resistance of isolated E.coli strains to aminoglycosides and quinolones antibiotics was serious.66%（66/100） of all the strains were positive with class Ⅰintegron and strain with class Ⅱintegon was not found.79 different genotypes were detected among the 100 stains.Conclusion Class Ⅰintegron could be widespread in E.coli isolates.ERIC-PCR could be a convenient and effective method for genotyping clinical E.coli isolates.
出处
《国际检验医学杂志》
CAS
2011年第12期1281-1282,共2页
International Journal of Laboratory Medicine
关键词
大肠杆菌
尿
整合子类
聚合酶链反应
escherichia coli
urine
integrons
polymerase charin reaction
