摘要
目的 观察金芪降糖片对糖尿病大鼠肾组织晚期糖化终末产物（AGEs）及其受体（RAGE）、核转录因子-κBp65（NF-κBp65）和单核细胞趋化蛋白1（MCP-1）表达的影响.方法 测定造模后20周糖尿病模型组（DM组）、糖尿病给药组（DD组）和正常对照组（N组）大鼠24 h尿微量白蛋白定量（UMA）、平均血糖、TG、TC、内生肌酐清除率（Ccr）水平及肾皮质AGEs、RAGE、NF-κBp65、MCP-1水平,同时观察各组大鼠肾脏形态学改变,评价金芪降糖片的肾脏保护作用.结果 DM组和DD组平均血糖、TC差异无统计学意义,DD组Ccr[（2.72±0.36）ml/min]高于DM组[（2.31±0.23）ml/min],而UMA、TG、AGEs、RAGE、NF-κBP65、MCP-1水平降低[（6.95±2.65）mg/d比（14.52±5.90）mg/d、（2.87±0.56）mmol/L比（4.35±0.92）mmol/L、（38.24±8.36）AFU/mg比（70.49±15.21）AFU/mg、（8.26±4.47）比（17.44±7.91）、（3.40±2.62）比（5.95±3.22）、（1.52±0.34）比（2.17±0.56）],差异有统计学意义（P＜0.05）;光镜和电镜下,DD组肾组织损伤程度均轻于DM组.结论 金芪降糖片可以减轻糖尿病慢性肾损伤的程度,其肾脏保护作用可能与其降脂及降低AGEs、RAGE、NF-κBp65、MCP-1水平有关.
Objective To observe the effect of jinqi tablet on the expression of advanced glycation end products（AGEs）, advanced glycation end products receptor（RAGE）, nuclear transcription factor（NF）-κBp65 and monocyte chemoattractant protein（MCP）-1 in the kidney of diabetic rats. Methods The level of AGEs, RAGE,NF-κBp65 NF-κBp65 and MCP-1 were determined in the kidney of diabetes group （group DM）, diabetes with jinqi tablet（group DD）and control group（group N）at the 20th week. The average plasma glucose（APG） ,triglyceride （TG） ,total cholesterol （TC）, endogenous creatinine clearance （Ccr）and urine microal bumin were collected.Results Compared with the group DM, the level of UMA, TG, AGEs, RAGE, NF-κBp65, MCP-1 in group DD was lower, but the Ccr was higher. The pathological changes in group DD were not as significant as that in DM group.Conclusions Jinqi tablet may decrease the renal damage of diabetes. The renal protection of jinqi tablet is not related to the control of blood sugar and it maybe related to the inhibition of AGEs, RAGE, NF-κBp65 and MCP-1.
出处
《中国医药》
2011年第9期1075-1078,共4页
China Medicine
