摘要
目的:观察针刺干预对脑心综合征(CCS)大鼠颈交感神经节去甲肾上腺素转运蛋白(NET)mRNA和心肌β1肾上腺素能受体(β1-AR)mRNA表达的变化,探讨CCS发病的交感通路机制以及针刺对其防治的作用机制。方法:Wistar大鼠18只随机分为伪手术对照组6只,其余采用胶原酶加肝素联合注射尾状核复制CCS模型。复制成功的大鼠12只再分为模型组和电针组各6只。电针组大鼠电针"水沟""风府""内关""心俞"穴,每次20 min,每天1次,连续3 d。于实验72 h后观察脑和心肌组织病理形态学变化;采用荧光定量PCR法检测各组大鼠颈交感神经节NET mRNA以及大鼠心肌β1-AR mRNA表达。结果:①伪手术组脑和心肌组织结构正常;模型组大鼠脑组织内可见大量红细胞,部分血细胞溶解,可见含铁血黄素沉积,血肿周围脑组织水肿,神经细胞肿胀,胞核碎裂溶解或消失,有大量炎性细胞浸润,心肌细胞变性、坏死,大量平滑肌断裂;电针组脑组织内红细胞明显少于模型组,脑组织轻度水肿,神经细胞变性坏死程度较轻,有少量炎性细胞浸润,心肌细胞变性、坏死程度较轻,仅有少量平滑肌断裂。②模型组NET mRNA相对表达量较伪手术对照组减少(P<0.01);电针组NET mRNA相对表达量较模型组增加(P<0.01)。③模型组β1-AR mRNA相对表达量较伪手术对照组减少(P<0.01);电针组β1-AR mRNA相对表达量较模型组增加(P<0.01)。结论:①颈交感神经节NET mRNA表达下调可能参与CCS的发生发展,针刺早期防治可拮抗这种变化,能有效阻止NET mRNA表达下调;②心肌β1-AR mRNA表达下调可能参与CCS的发生发展,针刺早期防治可能直接干预β1-AR mRNA表达下调,或干预其上游调控因素NET mRNA表达的下调。这些可能是针刺防治CCS交感通路作用机制之一。
Objective To observe the effect of electroacupuncture （EA） intervention on the expression of norepinephrine transporter （NET） mRNA in the middle cervical-stellate ganglion complex （MC-SGC） and βl-adrenergic receptor （131-AR） mRNA in the left ventricular myocardium in cerebral-cardiac syndrome （COS） rats, so as to study its underlying mechanism in the prevention and treatment of COS. Methods Eighteen Wistar rats were randomized into sham operation, model and EA groups （n =6）. COS model was duplicated by intravenous injection of 1 μL of saline containing collagenase （1 U/μL） ＋ heparin （7 U/μL） into the caudate nucleus. EA （2 Hz, 5 V） was applied to ＂Shuigou＂ （GV26）-＂Fengfu＂ （GV 16）, and ＂Neiguan＂ （PC 6）-＂Xinshu＂ （BL 15） for 20 min, once daily for 3 days. Seventy-two hours after modeling, pathological changes of the brain and cardiac tissueswere observed after sectioning and stained with HE method. The expression of MC-SGC NET mRNA and the myocardial β1-AR mRNA of the left ventricle were detected by fluorescence quantitative PCR. Results ①ln comparison with the model group, the number of the exuded red blood cells in the brain tissue around the caudate Nucleus region was fewer, the severity of the tissue edema, cell necrosis, and inflammatory cell infiltration lighter in the EA group. Compared with the model group, the severity of degeneration and necrosis of myocytes was lighter and the number of the broken cardiac smooth muscle fewer in the EA group. ②The expression levels of both MC-SGC NET mRNA and myocardial β1-AR mRNA were significantly lower in the model group than in the sham operation group（P〈0.01）. Compared with the model group, the expression levels of both MC-SGC NET mRNA and myocardial β1-AR mRNA were upregulated considerably in the EA group （P〈0.01）. Conclusion EA of GV 26-GV 16 and PC 6-BL 15 can upregulate the expression of both MC-SGC NET mRNA and myocardial β1-AR mRNA in CCS rats which may contribute to it
出处
《针刺研究》
CAS
CSCD
北大核心
2011年第4期252-257,共6页
Acupuncture Research
基金
安徽省自然科学基金项目(No.070413121)
安徽省人才开发专项资金项目(No.2008Z036)
