摘要
目的研究利用荧光分析识别生物粒子的实时监测技术和Andersen6级采样器采样对大气生物气溶胶采样结果的影响,为大气生物气溶胶监测方法提供理论依据。方法利用生物粒子实时监测采样仪采集大气生物气溶胶粒子,用Andersen6级采样器作为对照,比较两种方法采集的生物气溶胶粒子的浓度变化。结果实时监测采集到的生物粒子浓度为20~120个粒子（pts）/L,Andersen6级采样器检测到的微生物气溶胶菌落浓度集中在0.2~5cfu/L。实时监测每6s检测空气中生物粒子数,可实时、直观反映空气中粒子浓度的变化,而Andersen6级采样器检测结果是一定采样时间内的生物气溶胶的菌落浓度,需要较长的培养时间。两种采样方法检测到的大气生物气溶胶粒子浓度总的年度变化趋势基本一致,但是浓度值在不同采样时段分散性很大。结论实时监测采样灵敏度高,可及时反映生物粒子浓度的变化情况,比传统采样方法更适用于实时监测大气生物气溶胶本底变化规律,帮助判断是否存在生物气溶胶暴露的危害。
Objective To provide a theoretic basis for ambient bioaerosols monitoring by comparing the sampling results by real-time monitoring with fluorescence analysis technique and Andersen six-stage sampler.Methods The bioaerosols in the ambient air were collected by a biological particle real-time monitor,and simultaneously an Andersen six-stage sampler was used as a comparison.The concentration changes of bioaerosols were refleced by the results of the two sampling methods.Results The concentration of bioaerosols detected by real-time monitoring and Andersen six-stage sampler was 20-120 particles（pts）/L and 0.2-5 cfu/L,respectively.The real-time monitoring could directly reflect the concentration of airborne particles in real-time change,for it measured the particles concentration in the ambient air every six seconds while the sample from Andersen sampler needed to be cultured for a longer time.The total annual concentration of the atmospheric bioaerosol obtained by the two sampling methods changed in pretty much the same way,but the concentration values varied widely with time.Conclusion The real-time monitoring could reflect the real-time change of particle numbers with a high sensitivity and be more suitable for monitoring the background concentration of particles in the ambient air than the traditional Andersen sampling method.Morever,it could be useful in the risk assessment of potential bioaerosol exposure.
出处
《军事医学》
CAS
CSCD
北大核心
2011年第7期527-530,共4页
Military Medical Sciences
基金
国家自然科学基金资助项目(30700651)
