摘要
目的:弄清新鲜乌头草对小鼠的急性毒性。方法:野外采集新鲜乌头草,立即粉碎榨汁,得到新鲜乌头草汁,小鼠灌胃给药,测定新鲜乌头草的半致死量LD50,观察小鼠所出现的毒性反应,并测定小鼠血清的生化指标和心脏的氧化还原指标的变化。结果:以新鲜乌头草量计,乌头草对小鼠的LD50是7.550g/kg;乌头草高剂量组小鼠血清中丙氨酸氨基转移酶(ALT)、门冬氨酸转移酶(AST)、总蛋白(TP)、白蛋白(ALB)、尿素(Urea)、尿酸(Uric Acid)、肌酸激酶(CK)、α-羟丁酸脱氢酶(α-HBDH)、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)与对照组相比均有显著性升高;与对照组相比,心脏组织中丙二醛(MDA)含量显著升高、总抗氧化能力(T-AOC)显著降低,以及乳酸脱氢酶(LDH)活力显著降低。结论:新鲜乌头草有很大的急性毒性,主要毒性靶器官是心脏组织。
Objective：To explore the acute toxicity of fresh Caulis et Folium Aconitum carmichaeli Debx.in mice.Methods：The fresh Caulis et Folium Aconitum carmichaeli Debx.was shivered and pressed.The obtained juice were treated with a single oral dose,The medial lethal dose（LD50） of fresh Caulis et Folium Aconitum carmichaelii was determined in mice;the toxic reaction was observed;the serum samples were collected for biochemical analysis and the cardiac tissues were used to quantitated the redox state.Results：The LD50 of fresh Caulis et Folium Aconitum carmichaeli Debx was 7.550g/kg in mice.Compare to the control group,the activity of ALT,AST,CK,α-HBDH,LDH,CK-MB and the content of TP,ALB,Urea,Uric Acid were increased with significant difference in the mice serum of high dose fresh Caulis et Folium Aconitum carmichaeli Debx.group.The content of MDA increased,T-AOC decreased,and the vigor of LDH decreased significantly as compare to the control group.Conclusions：In general the fresh Caulis et Folium Aconitum carmichaeli Debx.could be considered to have acute toxicity,and the main target organ is cardiac tissue.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第8期1850-1852,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
新疆生产建设兵团科学技术局2009年度兵团科技计划(No.2009GG52)~~
关键词
乌头草
急性毒性
半数致死量
氧化还原
Aconitum carmichaelii herb
Acute toxicity
Medial lethal dose（LD50）
Redox
