摘要
目的 探讨川芎嗪及硫氮唑酮对照射后ＮＩＨ／３Ｔ３ 成纤维细胞增殖及ｂＦＧＦ表达的影响，为防治放射性肺纤维化的药物研究提供实验依据。方法 采用ＭＴＴ 法测定离体培养的ＮＩＨ／３Ｔ３ 成纤维细胞增殖程度并用免疫细胞化学ＡＢＣ 方法观察ｂＦＧＦ蛋白表达改变。结果 不同组ＭＴＴ法测得光密度值，对照组为０－７１ ±０－２２ ；照射组（Ⅰ） 为１－０３ ±０－２８ ；川芎嗪＋ 照射组为０－４７±０－１５ ；照射组（ Ⅱ） 为１－０５ ±０－２１；硫氮唑酮＋ 照射组为２－０２ ±０－２１；不同组ｂＦＧＦ 蛋白表达平均光密度：对照组为０－０２８±０－００５ ；照射组为０－０４４ ±０－００６ ；川芎嗪＋ 照射组为０－０２９ ±０－００１ ；硫氮唑酮＋ 照射组为０－０３６ ±０－００３。结论 川芎嗪明显抑制γ射线照射后ＮＩＨ／３Ｔ３ 成纤维细胞的增殖及ｂＦＧＦ蛋白表达，硫氮唑酮则不具有这一作用。
Objective\ In order to provide experimental basis for drug research of preventing and treating radiation pulmonary fibrosis,the authors observed the effect of ligustrazine and diltiazem on fibroblast proliferation and expression of basic fibroblast growth factor (bFGF) following 60 Co γ ray irradiation. Methods NIH/3T3 fibroblast proliferation and expression of bFGF were observed respectively by using MTT[3 (4,5 dimethylthiazo( 2 zyl) 2,5 diphenyl tetrazolium bromide)] method and ABC immunocytochemistry staining. Results The optical densities of bFGF were 0 71+0 22 for control group, 1 03+0 21 for irradiation group (Ⅰ), 0 47+0 15 for ligustrazine+irradiation group,1 05+0 21 for irradiation group (Ⅱ),and 2 02+0 21 for diltiazem+irradiation group.The mean optical densities of bFGF were 0 028+0 005,0 044+0 006,0 029+0 001,and 0 0036+0 003 for control group,irradiation group,ligustrazine+irradiation group,and diltiazem+irradiation group, respectvely. Conclusion Ligustrazine can inhibit the NIH/3T3 fibroblast proliferation and expression of bFGF,but diltiazem does not show such an effect.\;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1999年第6期379-380,共2页
Chinese Journal of Radiological Medicine and Protection
