摘要
为建立一种类似人类溃疡性结肠炎（UC）特点的动物模型，将60只封闭群健康成年Wistar大鼠随机分为3组造模，每组20只，A组应用2，4－二硝基氯苯（DNCB）和乙酸复合法，B组单纯应用DNCB，C组单纯应用乙酸，对3组进行对照研究。结果：病程：A组大于16周，B组2周，C组8周；免疫学反应：造模前及造模完成后1周CD4＋CD29＋细胞A组为5．01±2．01％，11．17±2．18％，P＜0．01；B组为4．96±1．87％，10．98±2．87％，P＜0．01；C组为4．93±1.96％，5．06±2．03％，P＞0．05；病理学改变：3种方法均可产生粘膜溃疡、炎性细胞浸润、隐窝脓肿等UC组织学特征；症状：3种方法均可造成大鼠腹泻、粘法血便等UC症状。结论：复合法大鼠UC模型既克服了DNCB病程短、自愈性强的缺点，也克服了乙酸法不具有免疫反应模型特征的缺点，又同时保留了二者症状、组织学改变与人类UC相似的优点，是一种较理想的新的UC模型。
Objective To establish a successful chronic ulcerative colitis (UC) animal model similiar as human.Methods:Sixty Wistar rats rats were divided into 3 group eg. A [combined 2,4-Dinitrochloro benzene (DNCB) and aceticacid(AA)],B(DNCB only),C(AA only). Results 1.The course of models were 16 weeks in group A,2. weeks in group B and 8 weeks in group C;2. Immunoreactivity: before and 1 week of the model was performed , the CD4 CD29cell were 5.01% + 2.01%, 11. 17% + 2. 18% in group A (P <0.01),4.96% + 1.87%, 10.98% + 2. 87% ingroup B (P< 0.01),4.93% + 1.96%,5 .06 + 2.03% in group C(P >0.05);3. Histology: mucosal congestion,netrophil infiltration,ulcer and abscess were observed in all three groups,Conclution.The UC model produced by combined DNCB and AA is successful and ressembles to human UC immunology in histologically.
出处
《青海医学院学报》
CAS
1999年第4期1-3,共3页
Journal of Qinghai Medical College
基金
济南军区总医院科研基金
