摘要
目的:本实验研究大鼠前脑缺血10 min后脑源性神经营养因子(brain-derived neurophic factor,BDNF)的高亲和力受体酪氨酸激酶B(tyrosine kinase B,TrkB)的抗体对海马内一氧化氮(nitric oxide,NO)产生的影响。方法:NO测定电极直接检测海马内NO浓度,海马内血流量使用激光多普勒血流测定仪监测。脑缺血前20 min通过微泵以0.08μl/min的速度向海马内持续输注相应液体。结果:脑缺血前对照组、生理盐水组及实验组间NO浓度、海马区血流量没有明显差异,脑缺血过程中3组在NO浓度、海马区血流量也没有显著差异,脑缺血再灌注后,实验组NO的浓度较对照组与生理盐水组产生显著减少。结论:受体TrkB抗体在脑缺血后显著降低海马内NO的产生。
Objective：To investigate the effects of anti brain-derived neurotrophic factor（BDNF） high affinity receptor tyrosine kinase B（TrkB） antibodies on nitric oxide（NO） concentration in rat hippocampus after forebrains ischemia.Methods：Hippocampal NO concentration was measured directly by an NO-selective electrode method,and hippocampal blood flow was measured with laser Doppler flowmetry.Liquid was continously infused at a rate of 0.08 μl / min by a microinfusion pump before 20 minutes of cerebral ischemia.Results：There was no significant diffence in NO concentration and the hippocampal blood flow among control group,physiological saline group and experimental group before and during cerebral ischemia.NO concentration of experimental group significantly decreased compared with that of control and saline group.Conclusion：Anti-TrkB antibody significantly reduced nitric oxide concentration in hippocampus after cerebral ischemia.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第8期1138-1141,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
杭州市科技发展计划医学重点专科专病项目资助(2005633Q21)
关键词
脑缺血
脑源性神经营养因子
一氧化氮
酪氨酸激酶B
cerebral ischemia； brain-derived neurotrophic factor； nitric oxide； tyrosine kinase B；
