摘要
目的:运用基质辅助激光解析电离时间飞行质谱技术(MALDI-TOF-MS)检测miR146a基因单核苷酸多态性,并探讨其与哈萨克族食管癌的相关性。方法:运用MALDI-TOF-MS检测261例哈族食管癌患者及190例同地区对照样本miR146a基因rs2910164位点单核苷酸多态性。结果:成功应用MALDI-TOF-MS检测了哈族食管癌miR146a基因rs2910164位点G/C多态,其基因型分布频率分别是36.4%,49.0%和14.6%,与对照组基因型分布频率(分别是36.3%,49.5%,14.2%)之间差异无统计学意义(P>0.05)。结论:基于MALDI-TOF-MS技术检测miR146a基因多态性的方法是一个快速、准确和可靠的检测方法。
Objective：Using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF MS） analysis to detect the single nucleotide polymorphisms of miR146a,and explorer the relationship between Hazakh＇s esophageal carcinoma and the single nucleotide polymorphisms of miR146a gene. Methods：Using MALDI-TOF MS to detect the SNP of 261 cases of Hazakh＇s esophageal squamous cell carcinomas（ESCC）,190 cases of cancer-free controls in the same area. Results：MALDI-TOF MS detected the SNP of miR146a sucessfully,miR146a gene rs2910164 G/C polymorphisms genotype frequencies were 36.4%,49.0% and 14.6%,the different has no statistic significant compare with the genes of control（36.3%,49.5%,14.2%）. Conclusion：the method based on MALDI-TOF-MS technique for detecting SNP miR146a gene is rapid,accurate and reliable method.
出处
《农垦医学》
2011年第2期118-121,共4页
Journal of Nongken Medicine
基金
国际科技合作与交流专项(2010DFB34100)
"十一五"国家科技支撑计划项目(2009BAI82B03)
