摘要
目的通过检测抗血管紧张素Ⅱ受体1型自身抗体（AT1-AA）在子痫前期患者外周血中的表达，探讨其在子痫前期发病中的病理生理机制。方法选择子痫前期患者30例，正常晚期妊娠患者20例，未孕健康妇女20例。以合成的抗血管紧张素Ⅱ受体为抗原，采用间接SA—ELISA法检测外周血AT1-AA的表达。结果子痫前期组外周血AT1-AA的阳性率为（65．0±4．7）％，明显高于正常晚期妊娠对照组（26．0±2．8）％和未孕健康妇女对照组（7．8±2．2）％，差异均有统计学意义（t值分别为24．97、38．56，P均〈0．01）。正常晚期妊娠对照组的阳性率与未孕健康妇女对照组比较差异也有统计学意义（t=4．58，P〈0．05）。结论子痫前期患者外周血中的抗AT1—AA高于正常孕妇和未孕健康妇女。提示AT1-AA在子痫前期发病中其重要作用；正常孕妇血清中抗AT1-AA的阳性率也髓显高于同龄未孕健康妇女，提示孕期有免疫机制的参与。
Objective To investigate the role of subtype 1 autoantibody against angiotensin Ⅱ receptor in the pathogenesis of preeclampsia by detecting its expression in the peripheral blood of preeclampsia patients, Methods Thirty patients with preeclampsia were assigned to preeclampsia group. Twenty normal pregnant women at the late stage and twenty non-pregnant healthy women as controls were investigated. The level of type 1 antoantibody against angiotensin Ⅱ in the peripheral blood was detected by indirect SA-ELISA assay with the produced ATR-1 as the antigen. Results The level of subtype 1 antoantibody against angiotensin Ⅱ（65 ± 4. 7） % in the peripheral blood of preeclampsia patients is significantly higher than that of normal late pregnant （ 26 ± 2. 8 ） % and non-pregnant women （7.8 ± 2. 2） % groups （ t1 = 24.97 ; t2 = 38.56 ; P 〈 0. 01 for both） ; The angiotensin ± receptor subtype 1 autoantibody in the group of normal late pregnancy （ 26 ± 2. 8 ） % was significantly higher than that of healthy non-pregnant women group （ 7. 8 ± 2. 2 ） % （ t = 4. 58, P 〈 0. 05 ）. Conclusion Compared with the normal pregnant women and the healthy non-pregant women, the autoantibody against AT1 receptor in sera of preeclamptic patients is elevated at a high frequency. These results suggest that overproduction of AT1-AA may play an important role during the development of preeclamptic patients. AT1-AA is a novel risk factor in pregnant women. Immune mechanisms may be involved in the process of pregnancy.
出处
《中国综合临床》
2011年第8期874-876,共3页
Clinical Medicine of China
