摘要
目的:探讨低分子肝素减轻急性百草枯中毒大鼠肺纤维化的可能作用机制。方法:55只雄性SD大鼠随机分成正常对照组5只,模型组25只,低分子肝素组25只。模型组与低分子肝素组禁食1 h后经口一次性给予百草枯75 mg/kg灌胃制成百草枯大鼠肺纤维化模型;低分子肝素组造模后皮下注射低分子肝素1 000 u/d,对照组与模型组给予0.2 mL/d生理盐水皮下注射。正常对照组于第1天,模型组、低分子肝素组于造模后1,3,7,14,28 d分别取5只大鼠肺组织行病理HE染色,取血清检测转化生长因子-β1、基质金属蛋白酶-9水平。结果:模型组血清转化生长因子-β1水平第3天明显升高;基质金属蛋白酶-9水平于第7天逐渐上升,第28天明显升高,与正常对照组比较差异均有统计学意义(P<0.05或P<0.01)。低分子肝素组第3天起转化生长因子-β1、基质金属蛋白酶-9水平较模型组降低(P<0.05或P<0.01)。病理结果示低分子肝素组肺泡壁出血减轻,肺纤维化程度较模型组减少。结论:低分子肝素可能通过影响转化生长因子-β1水平,减少基质金属蛋白酶-9的合成,减轻大鼠纤维化程度。
Objective To investigate the mechanism of low molecular weight heparin for relieving pulmonary fibrosis in rats with acute paraquat poisoning.Methods Fifty-five male SD rats were divided into control group（group A,n=5）,pulmonary fibrosis group（group B,n=25） and low molecular weight heparin prevention group（group C,n=25）.Pulmonary fibrosis was induced by feeding paraquat 75 mg/kg after fasting for one hour.Group C received subcutaneously low molecular weight heparin（1000 u/d）,and groups A and B received normal saline（0.2 mL/d）.Five rats in group B and C respectively were killed on the 3rd,7th,14th,28th days respectively,and specimens from lung tissue and serum were taken for HE staining.The serum transforming growth factor-β1 and matrix metalloproteinase-9 levels were measured.Results The level of serum transforming growth factor-β1 significantly increased on the third day in group B,and the level of serum matrix metalloproteinase-9 gradually increased in the 7th day and was significantly higher on the 28th day in group B,which was different from that in control group（P〈0.05,P〈0.01）.The transforming growth factor-β1,matrix metalloproteinase-9 levels decreased on the third day in group C compared with that in group B（P〈0.05,P〈0.01）.HE dyeing patho-observation showed bleeding and pulmonary fibrosis were relieved in group C compared with those in group B（P〈0.05）.Conclusion Low molecular weight heparin may reduce the synthesis of matrix metalloproteinase-9 and relieve fibrosis degree by influencing the level of transforming growth factor-β1 in rats.
出处
《中华实用诊断与治疗杂志》
2011年第8期766-768,共3页
Journal of Chinese Practical Diagnosis and Therapy
关键词
百草枯
中毒
低分子肝素
转化生长因子-Β1
Paraquat
poisoning
low molecular weight heparin
transforming growth factor-β1
