摘要
目的:探讨RUNX3基因启动子甲基化与非小细胞肺癌(NSCLC)预后的关系。方法:酚/氯仿法提取80例术后接受顺铂辅助化疗NSCLC患者的肺癌组织标本DNA,巢氏甲基化特异性PCR(nMSP)检测RUNX3启动子甲基化状态,并回顾性分析甲基化状态与临床病理特征、术后无病生存、总生存的关系。结果:80例NSCLC肿瘤样本中20例检测到RUNX3基因启动子甲基化(25.0%)。RUNX3启动子基因甲基化与病理类型相关(P=0.020),且腺癌(36%)高于鳞癌(11%)。N分期(OR:4.898,P<0.001)、RUNX3基因启动子甲基化(OR:20.293,P=0.011)是影响术后无病生存的独立危险因素。单因素K-M生存分析、Cox多因素分析表明RUNX3基因甲基化(RR:2.345,95%CI:1.130～4.865,P=0.022)是影响总生存的独立危险因素。结论:RUNX3基因甲基化是影响术后NSCLC无病生存和总生存的独立影响因素。
Objective To determine the relation between the promoter methylation status of RUNX3 gene and clinicopathological parameters,prognosis of non-small cell lung cancer（NSCLC）.Methods We collected 80 formalin-fixed paraffin-embedded lung cancer tissue samples from NSCLC patients who received postoperative adjuvant chemotherapy with cisplatin.Genomic DNA was extracted through phenol/chloroform extraction.The methylation status of RUNX3 was determined by nested methylation-specific PCR（nMSP）.We investigated the pathological and prognostic characteristics of NSCLC stratified by methylation status.Results The RUNX3 promoter methylation was observed in 20 of the 80 NSCLC samples（25.0%）.Methylation of RUNX3 was more frequent in adenocarcinomas（36%） than in squamous cell carcinomas（11%）（P=0.020）.In multivariate Logistic regression,positive RUNX3 methylation status（P=0.011） was found to be independent disease-free survival factor as was N stage（P0.001）.Kaplan-Meier curves showed patients with RUNX3 methylation had a significantly poorer overall survival than those without methylation（P=0.003;log-rank test）.In multivariate Cox proportional hazards regression analysis,RUNX3 methylation（RR：2.345,95% CI：1.30-4.865,P=0.022） was a significant independent prognostic factor for the overall survival.Conclusion RUNX3 methylation is a significant independent prognostic factor for disease-free survival and overall survival.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2011年第7期650-654,共5页
Journal of Central South University ：Medical Science
