摘要
目的:探讨miR-149在鼻咽癌中的功能和机制。方法:Real-time PCR和2-△△Ct计算方法验证miR-149在鼻咽癌细胞系中的表达,分别应用MTT实验、划痕实验和transwell迁移实验分析miR-149对鼻咽癌细胞的增殖、迁移和侵袭功能的影响。Western印迹检测E-cadherin的变化。结果:相对于正常鼻咽上皮细胞NP69,miR-149在5-8F和6-10B鼻咽癌细胞系中表达增高。MTT实验、划痕实验和transwell实验显示miR-149能够促进鼻咽癌细胞的增殖、迁移和侵袭。Western印迹证实miR-149能够降低E-cadherin的表达;反之,抑制miR-149能抑制鼻咽癌细胞的增殖、迁移和侵袭转移能力。结论:miR-149通过调节细胞上皮-间质转变在鼻咽癌的侵袭转移中发挥重要作用。
Objective To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma（NPC）.Methods The expression of miR-149 was examined by real-time PCR and calculated by 2-△△Ct method.The cell proliferation was analyzed by MTT assay.The cell migration and invasion were shown by the wound healing assay and transwell migration assay,and the expression of E-cadherin was detected by Western blot.Results The expression of miR-149 was higher in NPC cell lines 5-8F and 6-10B than that in normal immortalized nasopharyngeal epithelial NP69.MTT assay showed that miR-149 promoted the proliferation of NPC cell lines.The wound healing assay showed miR-149 promoted the mobility and invasion of NPC cell lines.Inhibition of miR-149 reduced the ability of NPC cell lines to proliferate and invade.miR-149 downregulated the expression of E-cadherin,whereas antagomir which mediated knockdown of miR-149 significantly upregulated the expression of E-cadherin.Conclusion miR-149 might be involved in the invasion and metastasis of NPC through regulation of epithelial-mesenchymal transition（EMT）.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2011年第7期604-609,共6页
Journal of Central South University ：Medical Science
基金
supported by the National Natural Science Foundation of China(81000882,81000972)
关键词
miR-149
鼻咽癌
迁移
侵袭转移
上皮-间质转变
miR-149
NPC
cell mobility
invasion and metastasis
epithelial-mesenchymal transition
