摘要
生物体内的蛋氨酸合成酶(methionine synthase)以N^5-甲基四氢叶酸作为辅酶,通过两步SN2反应,将甲基转移到高半胱氨酸的流基上生成蛋氨酸。该酶起到中间甲基载体的功能。为了进一步研究甲基转移的机理,报道了作为活化的5-甲基四氢叶酸模型:碘化2-氨基-4-羟基-5,5,6-三甲基吡啶并[3,2-d]嘧啶的合成以及与模拟蛋氨酸合成酶[Co(I)(dmgH)_2Py]的反应。
As a model compound in methyl transfer process from TV5 - methyltetra - hydrofolate (III) to methionine synthase, 2 - amino - 4 - hydroxy -5,5,6- trimethyl -5,6,7,8- tetrahydropyrido [3,2-d ] pyrimidinium iodide 8 was synthesized from 5 - aminouacil through seven stepsof reactions. Compound 8 reacts with [Co(I) (dmgH)2Py] to give CH3 - Co(dmgH)2Py. The structures of all compounds were characterized by elemental analysis,UV,1H NMR and MS.
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
1999年第6期582-586,共5页
Chinese Journal of Organic Chemistry
关键词
甲基四氢叶酸
蛋氨酸合成酶
甲基转移
辅酶
模拟
methionine synthase, methyl transfer, coenzyme model, pyrido [ 3,2 - d ] pyrimidine
