摘要
目的探讨环氧化酶（COXs）在神经病理性痛大鼠背根神经节P2X，受体表达上调中的作用。方法雄性SD大鼠24只，体重250～280g，采用随机数字表法，将其随机分为4组（n=6），神经病理性痛组（CCI组）、COX-1抑制剂组（I组）和COX-2抑制剂组（C组）制备坐骨神经慢性缩窄性损伤（CCI）模型，假手术组（S组）仅暴露坐骨神经。于术后3．14dI组和C组分别以COX-1抑制剂布洛芬40mg·kg^-1·d^-1和COX-2抑制剂塞来昔布30mg·kg^-1·d^-1灌胃。分别于术前（基础状态）、术后3、5、7A0、14d时测定热缩足潜伏期（PWL）和机械缩足阚值（PWT）。然后处死大鼠，取L1-15节段背根神经节，测定P2X受体mRNA及其蛋白表达水平。结果与S组比较，CCI组术后PWL缩短，PWT降低，P2X受体mRNA及其蛋白表达上调（P〈0．05）；与CCI组比较，I组和C组术后PWL延长，PWT升高，P2墨受体mRNA及其蛋白表达下凋（P〈0．05）；与I组比较，C组术后PWL延长，邢汀升高，背根神经节P2X受体mRNA及其蛋白表达上调（P〈0．05）。结论COXs参与了神经病理性痛大鼠背根神经节P2X1受体表达上调，且COX-1的作用强于COX-2。
Objective To investigate the role of cyclooxygenases （COXs） in the up-regulation of the expression of P2X3 receptors in the dorsal root ganglion （DRG） in rats with neuropathic pain. Methods Twenty-four male SD rats, weighing 250-280 g, were randomly divided into 4 groups （ n = 6 each） ： sham operation group （group S）, chronic constrictive injury （CCI） group, COX-1 inhibitor ibuprofen group （group I）, and COX-2 inhibitor celecoxib group （group C）. Neuropathic pain was induced by CCI. The animals were anesthetized with in- traperitoneal 10% chloral hydrate 300-500 mg/kg. CCI was produced by placing 4 ligatures on the left sciatic nerve at 1 mm intervals. In group S, the left sciatic nerve was only exposed but not ligated. In groups I and C, ibuprofen 40 mg·kg^-1·d^-1 and celecoxib 30 mg·kg^-1·d^-1 were given through a gastric tube into the stomach at day 3-14 after operation respectively. Paw withdrawal latency （PWL） and paw withdrawal threshold （PWT） were measured before operation （baseline）, and at 3, 5, 7, 10 and 14 days after operation. Then the rats were sacrificed and their L4.6 DRGs were removed to detect the expression of P2X3 mRNA and protein. Results Compared with group S, PWL was significantly shortened, PWT decreased, and P2X3 mRNA and protein cxprcssion up-regulated in group CCI （P 〈 0.05）. Compared with group CCI, PWL was significantly prolonged, PWT increased, and P2X3 mRNA and protein expression down-regulated in groups I and C （P 〈 0.05 ）. Compared with group I, PWL was significantly prolonged, PWT increased, and P2X3 mRNA and protein expression up-regulated in group C （ P 〈 0.05）. Conclusion COXs are involved in the up-regulation of the expression of P2X3 receptors in the DRG in rats with neuropathic pain, and the effect of COX-1 is stronger than that of COX-2.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第6期702-705,共4页
Chinese Journal of Anesthesiology
基金
湖南省博士生科研刨新项目（CX20108089）
中南大学优博扶植基金
