摘要
为利用康氏木霉(Trichoderma.koningii)降解稻草提供工艺参数,以康氏木霉(T.koningii)N18为菌株,进行了固体发酵产纤维素酶条件的优化。确定了康氏木霉(T.koningii)的最佳固体发酵培养条件:稻草:麸皮的最佳比例为6:2,最佳氮源为(NH)SO,碳氮比为6:1,含水量为200%,最适产酶pH为6.0,最佳产酶温度为28℃,最佳产酶时间为7d,康氏木霉(T.koningii)所产纤维素酶各组分酶活力分别为:羧甲基纤维素酶活力为321.64U.mL,滤纸分解酶活力为59.58U.mL。4 2
The culture conditions for the production of cellulases was optimized by using Trichoderma.koningii N 18.Results indicated that the highest level of Solid fermentation occurred in the optimal ration of straw and bran 6：2,and ammonium sulfate was the best nitrogen source with the optimal ratio of C：N 6：1.And the water content was 200% with the optimal conditions of enzyme pH 6.0.The optimal culture temperature was 28 ℃,and the optimal time was 7d.Under the optimal culture condition,the activities of CMcase and FPAase were 321.64U·mL^-1 and 59.58 U·mL^-1,respectively.
出处
《中国西部科技》
2011年第23期1-3,共3页
Science and Technology of West China
关键词
康氏木霉
固体发酵
纤维素酶
发酵条件优化
Trichoderma koningii
Solid fermentation
Cellulose
Optimal fermentation conditions
