摘要
为了解瑞士乳杆菌L7(Lactobacillus helveticusL7)洗涤菌体生物合成共轭亚油酸(CLA)的能力,通过单因素和正交优化研究,确定了瑞士乳杆菌L7洗涤菌体合成c9,t11-CLA的最适条件:0.05mol/LPBS缓冲液(pH5.8)、1.00mg/mL亚油酸、23℃反应12h。在最适转化条件下,c9,t11-CLA产量达到0.54mg/mL。结果表明,洗涤菌体合成CLA的产量和分批发酵相近,可以继续进行瑞士乳杆菌L7固定化细胞发酵生产CLA的研究。
For the purpose of producing c9,t11-conjugated linoleic acid（CLA） by washed cells of Lactobacillus helveticus L7,the bioconversion of c9,t11-CLA by washed cells of L.helveticus L7 was optimized.It was found that the optimum conditions were 0.05 mol/L PBS buffer（pH5.8） with 1.00 mg/mL linoleic acid,and incubated at 23 ℃ for 12 h.The yield of c9,t11-CLA was up to 0.54 mg/mL by washed cells of L.helveticus L7 in the optimum conditions.It showed that the yield is the same as the batch fermentation,further work can be continued on immobilized cell fermentation.
出处
《食品科技》
CAS
北大核心
2011年第8期8-11,共4页
Food Science and Technology
基金
江西省工业科技支撑重点计划项目(2009S00746)
关键词
瑞士乳杆菌L7
共轭亚油酸
生物合成
L.helveticus L7
conjugated linoleic acid（CLA）
bioconversion
