摘要
目的探求一种简易、经济的小鼠原代肝细胞的分离与培养方法。方法采用非灌注法分离小鼠肝脏,利用0.2%Ⅳ型胶原酶对肝脏消化以获取肝细胞,以DMEM培养基对肝细胞进行单层培养。结果光镜下可见培养的肝细胞呈三角形、圆形、类圆形或扁平不规则多角形,排列整齐,细胞界限清晰,胞浆丰富透亮,细胞中有核,有单核、双核是圆形或椭圆形,核仁清晰。结论此方法较适合一般实验室开展小鼠原代肝细胞的分离与培养,为进一步的实验研究提供了基础。
Objective To search for an easy and cheap method for the culture of murine primary hepatocytes. Methods The murine liver was isolated by non-perfusion, digested by 0.2% Ⅳ-Collagenase so as to get the primary hepatocytes and culture them with DMEM medium in the monolayer. Results Liver cells were found under the optical microscope, showing as triangle, roundness, and so on. The cells was marshalling, and cell boundary was distinct. Cytoplasm was abundant and bright. There were one or two circular nucleuses in each cell. Conclusion This method was good for the general labs to culture the hepatocytes, and it can provide a good basic for further research.
出处
《内科》
2011年第5期400-402,共3页
Internal Medicine
关键词
原代细胞培养
肝细胞
细胞分离
细胞培养
Culture of primary ceils
Hepatocytes
Cell Isolation
Cell Culture
