摘要
目的和方法：采用ＡＢＣ免疫组织化学法结合图象分析，观察大鼠脑组织神经肽Ｙ、亮氨酸脑啡肽、强啡肽Ａ１１３ 在长期（ 共７ 周）大强度（速度由１５ ｍ／ｍｉｎ 递增至３５ ｍ／ｍｉｎ、运动时间为２０ ～２５ ｍｉｎ／ｄ） 的运动下的变化。结果：安静状态下在丘脑室旁核（ＰＶ） 、下丘脑背内侧核（ＤＭ） 、下丘脑腹内侧核（ＶＭＨ）等核团ＮＰＹ 无显著性变化；在此基础上的末次急性运动结束后３ ｈ ＮＰＹ 变化尤为明显。安静状态下大鼠尾壳核ＬＥＮＫ 下降；而末次急性运动后大鼠下丘脑ＬＥＮＫ 被迅速激活而升高。该强度运动能激活下丘脑ＤＹＮＡ１１３ ，尤以运动结束后３０ ｍｉｎ 最为明显。结论： ＮＰＹ、ＬＥＮＫ、ＤＹＮＡ１１３
Aim and Methods:To study the mechanism of long term (total 7 weeks) and high intensity exercise (speed from 15 m/min gradually increased to 35 m/min, 20 to 25 min/d duration)on changes of NPY, L ENK and DYN A 1 13 in different areas of rat brain. The method of avidin biotin complex immunohistochemistry integrating with image pattern analysis was adopted.Results:In such intensity exercise, the rats brain NPY was unchanged in paraventricular thalamic (PV), dorsomedial hypothalamic nucleus (DM) and ventromedial hypothalamic nuclues (VMH) at their pre exercise state, but it was changed obviously especially at 3 hours post exercise after final exercise. L ENK was decreased in caudate putamen at their pre exercise state, but it was activated with sharply increased after final exercise. DYN A 1 13 in hypothalama could be activated by such exercise, especially at 30 minutes post exercise. Conclusion: The dynamic changes of NPY,L ENK and DYN A 1 13 in different areas of rat brain were not the same. To study the mechanism of long term (total 7 weeks) and high intensity exercise (speed from 15 m/min gradually increased to 35 m/min, 20 to 25 min/d duration)on changes of NPY, L ENK and DYN A 1 13 in different areas of rat brain. The method of avidin biotin complex immunohistochemistry integrating with image pattern analysis was adopted.Results:In such intensity exercise, the rats brain NPY was unchanged in paraventricular thalamic (PV), dorsomedial hypothalamic nucleus (DM) and ventromedial hypothalamic nuclues (VMH) at their pre exercise state, but it was changed obviously especially at 3 hours post exercise after final exercise. L ENK was decreased in caudate putamen at their pre exercise state, but it was activated with sharply increased after final exercise. DYN A 1 13 in hypothalama could be activated by such exercise, especially at 30 minutes post exercise. Conclusion: The dynamic changes of NPY,L ENK and DYN A 1 13 in different areas of rat brain were not
出处
《中国应用生理学杂志》
CAS
CSCD
1999年第4期306-309,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
关键词
运动性疲劳
神经肽Y
亮氨酸脑啡肽
强啡肽
exercise induced fatigue
neuropeptide Y
leucine enkeplialin
dynorphin A_(1-13)
immunohistochemistry
