摘要
克隆到猪Jiv90基因,构建原核表达载体并在大肠埃希菌中表达。从猪血管内皮细胞中克隆到Jiv90基因,将其克隆到pET-32a载体上,构建出重组表达载体,经IPTG诱导表达。结果表明,本试验成功克隆了大小为693 bp的基因,重组质载体pET-32a-Jiv90所表达的蛋白在大肠埃希菌中以包涵体的形式存在,表达产物经SDS-PAGE和Western blot鉴定大小为46 ku,与预期结果一致。为进一步研究该蛋白的功能及制备抗体奠定了基础。
The research studied the cloning and expression of Jiv90 gene.693 bp of Jiv90 gene from swine vein endothelia cell was amplified by PCR,and cloned into prokaryotic expression vector pET-32a,and expressed in E.coli BL21（DE3）.The target protein was expressed in E.coli BL21（DE3） induced with IPTG,and detected by SDS-PAGE and Western blot.The results indicated that the 46 ku expressed protein could be recognized by mouse antiHis-tag antibody.The results laid the foundation for studies on the protein function and the preparation of antibodies.
出处
《动物医学进展》
CSCD
北大核心
2011年第7期9-13,共5页
Progress In Veterinary Medicine
基金
国家转基因生物新品种培育重大专项-抗病转基因新品种培育(2009ZX08006-006B)
陕西省"13115"科技创新工程重大科技专项-猪瘟病毒致病机理与抗病育种研究(2009ZDKG-23)
