摘要
目的测定麦冬药材中甲基麦冬黄烷酮A、甲基麦冬黄烷酮B和6-醛基异麦冬黄烷酮A的含量,为麦冬药材的质量控制提供科学依据。方法采用Waters Symmetry C18柱(4.6 mm×250 mm,5μm),乙腈-0.05%磷酸梯度洗脱,流速为1.0 mL.min-1,检测波长296 nm,柱温30℃。结果甲基麦冬黄烷酮A、甲基麦冬黄烷酮B和6-醛基异麦冬黄烷酮A分别在9.68～96.8、4.96～49.6、4.18～41.8μg.mL-1内具有良好的线性关系,平均回收率(n=6)分别为100.1%(RSD 1.03%),99.5%(RSD 1.35%)和100.2%(RSD 1.16%)。结论该方法快速、准确,可用于麦冬药材中甲基麦冬黄烷酮A、甲基麦冬黄烷酮B和6-醛基异麦冬黄烷酮A的含量测定。
OBJECTIVE To determine the contents of methylophiopogonanone A, methylophiopogonanone B and 6-aldehydo-isoophiopogonone A in Radix Ophiopogonis. METHODS Waters Symmetry C ls column （4. 6 mm x 250 mm, 5 μm） was used with phosphoric acid （0. 05%, v/v） and acetonitrile as the mobile phase. Stepwise gradient elution was adopted. The flow rate was 1.0 mL·min^-1, the detection wavelength was 296 nm, and the column temperature was maintained at 30 ℃. RESULTS The calibration curves showed good linearity within the range of 9. 68 -96. 8, 4. 96 -49. 6 and 4. 18 -41.8 μg ·min^-1for methylophiopogonanone A, methylophiopogonanone B and 6-aldehydo-isoophiopogonone A, respectively. The mean recoveries （ n = 6 ） were 100. 1% （ RSD 1.03% ）, 99. 5% （RSD 1.35% ） and 100. 2% （ RSD 1.16% ）, respectively. CONCLUSION This method is simple and accurate. And it can be used for the determination of methylophiopogonanone A, methylophiopogonanone B and 6-aldehydo-isoophiopogonone A in Radix Ophiopogonis.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2011年第15期1209-1211,共3页
Chinese Pharmaceutical Journal
基金
"国家重大新药创制"科技重大专项(2010ZX09502-004)
科技创新专项资金(08FDZDSH01404)
