摘要
目的探讨肺癌患者胸腔积液及外周血CD＋4CD＋25调节T细胞、T细胞亚群的特点及其临床意义。方法采用流式细胞术检测68例肺癌患者外周血和其中32例并发胸腔积液患者的胸腔积液及56名健康对照者外周血中CD＋4CD＋25T细胞和淋巴细胞亚群的水平。结果不同TNM分期肺癌患者外周血T淋巴细胞亚群表达比例不同，其中Ⅰ＋Ⅱ、Ⅲ、Ⅳ期患者外周血CDIcD击细胞占cD刍细胞比例分别为（19．52±3．32）％、（27．28±8．26）％、（32．31±15．60）％，均高于健康对照组的（11．12±3．32）％，差异均有统计学意义（t=31．0040、-7．9688、-4．9770，均P〈005）。合并胸腔积液患者胸腔积液CDICD嘉细胞占CD＋34细胞比例为（34．12±18．63）％，高于其外周血的（26．36±16．25）％，差异有统计学意义（t=21．164，P〈0．05）；合并胸腔积液肺癌患者胸腔积液及外周血CD＋4细胞比例分别为（25．32±13．45）％及（34．68±12．34）％，低于健康对照组的（43．24±8．68）％（t=7．3104、4．8818，均P〈0．05），CD＋56细胞比例分别为（8．24±7．38）％及（11．23±7．65）％，低于健康对照组的（18．23±9．23）％（t=-14．7549、-11．7216，均P〈0．05），CD＋4／CD＋8分别为（1．02±0．56）％及（1．32±0．82）％，低于健康对照组的（1．89±0．32）％，差异均有统计学意义（CD＋4／CD＋8;t=-24．78、-4．4564，均19〈0．05）。结论肺癌患者胸腔积液及外周血CD＋4CD＋25T细胞水平升高，与肿瘤患者免疫功能低下及肿瘤的发生、发展密切相关。
Objective To explore the characteristics and clinical significance of CD＋4CD＋25 Regulatory T lymphocytes and T cell subsets in peripheral blood and malignant pleural effusion from lung cancer patients. Methods Flow cytometry was used to detect the percentage of CD＋4 CD＋25 regulatory T cells and T cell subsets in peripheral blood from 68 lung cancer patients and 56 healthy persons, and in pleural effusion from 32 lung cancer patients with malignant effusion. Results T lymphocyte subsets in peripheral blood of lung cancer patients in different periods were expressed differently. The percentage of CD＋4CD＋25 regulatory T cells in peripheral blood were （19.52±3.32） %, （27.28±8.26） % and （32.31±15.60） % in Ⅰ ＋ Ⅱ, Ⅲ and Ⅳ period lung cancer patients, respectively, and were higher than that of healthy volunteers （11.12±3.32） % （t =31.0040, -7.9688, -4.9770, P 〈0.05）. In the lung cancer patients with malignant effusion, the percentage of CD＋4 CD＋25 regulatory T cells in the pleural effusion was higher than that in the peripheral blood [（34.12±18.63） % vs （26.36±16.25） %, t =21.164, P 〈0.05]. In the lung cancer patients with malignant effusion ,the percentages of CD＋4 in peripheral blood and pleural effusion were （25.32±13.45） % and （34.68±12.34） %, were lower than that in healthy volunteers （t =7.3104, 4.8818, P 〈0.05）, the percentages of CD;6 were （8.24±7.38） % and（ll.23-＋ 7.65） %, CD＋4/CD＋8 were （1.02±0.56） % and （1.32±0.82） %, were lower than （18.23±9.23） % and （1.89±0.32） % in healthy volunteers, respectively, （CD＋56： t =-14.7549, -11.7216; CD＋4/CD＋8： t =-24.78, -4.4564, P 〈0.05）. Conclusion The relative increase of CD＋4 CD＋25 Regulatory T cells may be related to immunosuppression and tumor progression in patients with lung cancer. Conclusion The relative increase of CD＋4CD＋25 regulatory T cells may be related to immunosuppression and tumor progression in patients with lung cance
出处
《肿瘤研究与临床》
CAS
2011年第7期463-466,共4页
Cancer Research and Clinic
基金
山东省教育厅科技计划（JK06L04）
