摘要
目的运用大孔吸附树脂分离纯化桔梗总皂苷,为桔梗总皂苷的生产提供参考。方法采用单因素试验结合正交试验法,以桔梗总皂苷含量、保留率为指标,对桔梗总皂苷的纯化工艺进行优选。结果最佳条件为选用D101型大孔树脂,上样浓度0.5g生药材/ml,径高比1:9,吸附流速为2BV/h,吸附2h后,以6BV水洗脱,再分别以5BV25%、70%乙醇洗脱,解析流速为3BV/h,收集70%乙醇洗脱液,纯化后桔梗总皂苷的含量达到56.32%,保留率为74.86%。结论 D101树脂对桔梗总皂苷具有较好的吸附和解析性能,纯化工艺简单可行、重复性好,具有良好的产业化前景。
Objective Purification of platycodins by macroreticular resin to provide evidence for their industrialized production. Methods A reasonable purifying process was established by a combination of single-factor experiments and orthogonal designs, using the content and retention rate of platycodins as the evaluation makers. Results The optimum conditions were as follows： the type of macroreticular resin was D101, the concentration of loading sample was 0.5g/ml, the ratio of diameter-length was 1：9, the absorbing velocity of flow was 2BV/h, After an absorption period of 2 hours, the column was eluted with 5BV water and then with 5BV 25%, 70%ethanol at a flow rate of 3BV/h, respectively, collected the 70% ethanol eluent, after purification of platycodins by macroreticular resin, the content of platycodins was 56.32% and the retention rate was 74.86%. Conclusion D101 macroreticular resin had good properties of absorption and resolution for platycodins, the process was simple and reproducible, with good prospects for industrial.
出处
《中国中医药现代远程教育》
2011年第9期153-155,共3页
Chinese Medicine Modern Distance Education of China
