摘要
以NaCl为选择因子,对长期继代培养的紫花苜蓿愈伤组织进行选择,筛选出耐1.2%NaCl的耐盐细胞系并对其进行生理分析,结果表明:耐盐细胞系对逆境的忍受能力高于对照,积累的脯氨酸和可溶性蛋白含量也高于对照,维持着较高的K+/Na+比,在盐分胁迫下能维持较高的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性。
Calluses of Southern type alfalfa cultured for a long term were selected,a cell line tolerant to 1.2% NaCl was isolated by using NaCl as selection pressure.The physiological and biochemical properties of this salt tolerant cell line were analyzed.The results showed that the tolerance of the salt-tolerant cell line was much higher than that of control line.Free proline content and soluble protein content increased in salt-tolerant cell line was higher than that in control,a higher K＋/Na＋ ratio was kept in the salt-tolerant cell line.The activities of superoxide dismutase（SOD）,peroxidase（POD） in salt-tolerant cell line were higher than that of the control under NaCl stress.
出处
《中国草地学报》
CSCD
北大核心
2011年第4期68-72,共5页
Chinese Journal of Grassland
基金
浙江省自然科学基金"耐南方滩涂种植的紫花苜蓿细胞突变体筛选及机理研究"(Y304072)
"南方型紫花苜蓿耐盐突变体盐胁迫特异蛋白及相关基因筛选与鉴定"(Y3090498)
国家"十二五"科技支撑计划项目"中东部地区优质紫花苜蓿高效生产利用关键技术研究与集成示范"(2011BAD17B04)
关键词
南方型紫花苜蓿
耐盐细胞系
筛选
生理特性
Southern type Medicago sativa
Salt-tolerance cell line
Selection
Physiological characterization
