摘要
文中观察了一株产脂肪酶毛霉(Mucor sp.)菌株在橄榄油罗丹明B鉴定平板上的菌落形态,对其产脂肪酶进行了酶学特性研究,并探讨了该菌株产脂肪酶的发酵条件,优化了产酶发酵培养基和产酶发酵条件,得出了该菌产酶的最佳发酵条件为以1%全脂大豆粉为氮源,1%玉米浆为碳源,添加0.5%橄榄油和0.1%Tween-80,控制起始pH值为6.0。
The colonial morphology of lipase-producing Mucor sp. on olive oil Rhodamine B agar plate was observed. The enzymatic characteristics of the lipase produced by Mucor sp. were studied, as well as the fermentation conditions for lipase production by Mucor sp.. The fermentation medium and conditions were optimized as follows： 1% full-fat soybean powder was used as nitrogen source, 1% corn syrup was used as carbon source, 0,5% （v/v） olive oil and 0.1% Tween-80 were added, and the initial pH value was 6.0.
出处
《中国酿造》
CAS
北大核心
2011年第7期134-137,共4页
China Brewing
关键词
毛霉
脂肪酶
发酵条件
Mucor
lipase
fermentation conditions
