摘要
以3个水稻品种成熟胚诱导的良好胚性愈伤组织为受体,以LycB为目的基因,应用根癌农杆菌介导法对水稻进行遗传转化,同时以抗性愈伤率为依据,对影响转化的几个因素进行优化研究。结果表明,预培养4d、侵染5-10min、农杆菌菌液浓度OD600值0.7-1.0、共培养2d有利于提高转化率。经潮霉素筛选获得的抗性植株经PCR和PCR-Southern分析鉴定,初步证明外源基因LycB已整合到水稻的基因组中。
Callus induced from mature embryos of 3 rice varieties were transformed by Agrobactorium tumefaciens.Transformation efficiency was affected by factors of pre-culture time,density of bacteria,duration for inoculation and co-cultivation.The transformation conditions were optimized with the combination of pre-culture of 4 days,infection in bacterium with OD600 0.7—1.0 for 5—10 minutes,followed by co-culture of 2 days.Plantlets with resistance to hygromycin were regenerated.PCR and PCR-Southern analysis on them confirmed the integration of the LycB be into the rice genome.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2011年第4期605-611,共7页
Journal of Plant Genetic Resources
基金
国家植物转基因技术研究开发与中试基地建设专项(J99-B-001)
国家转基因生物新品种培育重大专项(2009ZX08003-019B
2008ZX08003-005
2008ZX08004-001)
