摘要
目的探讨雌激素减轻实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)炎症反应的可能机制。方法用MOG35-55多肽诱发60只EAE小鼠模型,做去卵巢术。分为治疗组(n=30)和对照组(n=30),治疗组予雌激素治疗。比较两组EAE小鼠的临床症状评分。取脑和脊髓,行H-E染色观察病理学改变,实时荧光定量PCR及ELISA检测EAE小鼠CNS中白细胞介素(interleukin,IL)-17、IL-23、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、干扰素γ(interferonγ,IFN-γ)、IL-4水平。结果治疗组EAE小鼠与对照组相比临床症状减轻(P<0.05);H-E染色显示治疗组炎细胞浸润减少(P<0.05),实时荧光定量PCR及ELISA结果示治疗组CNS中IL-17、IL-23、TNF-α、IFN-γ表达降低而IL-4增加(P<0.05)。结论雌激素可能通过降低IL-17、IL-23、TNF-α、IFN-γ,增加IL-4,从而减轻EAE小鼠炎症反应。
Objective To explore the anti-inflammatory mechanisms of estrogen on experimental autoimmune encephalomyelitis（EAE）. Methods 60 mouse EAE models were induced with MOG35-55 peptide, all animal ovarectomized. They were divided into treatment group and control group. Treatment group was treated by estrogen. Two groups were compared by clinical symptom scores. The pathologic changes of the brain and spinal cord were studied by H-E stai- ning. The expression of interleukin-17 （ IL-17）, IL-23, tumor necrosis tactor α（TNF-α）, interferon γ（ IFN-γ）, IL-4 mRNA and protein were examined by quantitative real-time PCR and ELISA. Results Clinical symptom score of treatment group were lower than that in the control group（ P 〈 0.05 ）. H-E staining showed that infihration of inflammatory cell decreased in treatment group（P 〈 0. 05 ）. The result of quantitative real-time PCR and ELISA showed the expression of IL-17, IL-23, TNF-α, IFN-γ were decreased and IL-4 was increased（P 〈 0. 05）. Conclusions Estrogen could decrease IL-17, 11,-23, TNF-α, IFN-γ and increase IL-4 to reduce inflammation of EAE mice.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2011年第7期396-400,共5页
Chinese Journal of Nervous and Mental Diseases
基金
贵州省科技厅攻关项目(编号:黔科合sy[2009]3054)
贵州省优秀科技教育人才省长专项资金项目(编号:黔省专合字(2010)86号)
