摘要
背景:膜超滤具有对蛋白质天然构象破坏较小以及回收率较高的特性,被应用于蛋白质的分离及纯化上。目的:利用所建立的传质模型优化血红蛋白超滤操作条件以分离出高纯度的血红蛋白。方法:利用膜超滤分离纯化的方法,研究浓缩血红蛋白溶液在超滤过程中溶液水分的渗透通量,溶液浓度和滤膜内外压力差之间的相互关系,对膜阻力和浓差极化阻力进行了定量描述,建立传质模型。结果与结论:①在压力为0.04MPa,温度为12℃条件下,溶液浓度分别为1.18,1.30,1.39,1.56g/L时,溶液的超滤渗透通量分别1.9375,1.8750,1.8124,1.7500L/(m2·h)。②在相同单位时间条件下,压力为0.02,0.03,0.04,0.05,0.06MPa时,溶液的超滤渗透通量为0.165,0.245,0.325,0.400,0.475L/(m2?h)。③试验的膜阻力Rm为0.1182,压力系数a等于0.128。随着蛋白浓度逐步升高,凝胶层逐渐形成,当达到凝胶浓度后再浓缩较困难。在试验条件下血红蛋白溶液凝胶浓度Cg为18.82g/L,超滤传质系数k为0.7008。
BACKGROUND：Ultrafiltration membrane has the characteristics of little destruction of the natural conformation of the protein and high recovery,which has been used in the isolation and purification of protein.OBJECTIVE：To optimize utrafiltration of hemoglobin by using of the mass transfer model in order to isolate highly purified hemoglobin.METHODS：Penetration flux,concentration of solution and the relationship between pressure and flux of enrichment hemoglobin solution in the process of ultrafiltration were studied by membrane ultrafiltration isolation and purification methods.Membrane resistance and concentration polarization resistance were quantitatively described and the mass transfer model was established.RESULTS AND CONCLUSION：①For the solution concentration of 1.18,1.30,1.39,1.56 g/L,the ultrafiltration flux was 1.937 5,1.875 0,1.812 4,1.750 0 L/（m2·h） respectively at the pressure of 0.04 MPa,12 ℃;②For the different pressure：0.02,0.03,0.04,0.05,0.06 MPa,the ultrafiltration flux was 0.165,0.245,0.325,0.400,0.475 L/（m2·h） respectively with the same time;③The membrane resistance was 0.118 2,and the pressure coefficient was 0.128.However,the gel layer was gradually formed with the increase of protein concentration;it was hard to concentrate the hemoglobin when the concentration was equal to the gel concentration.The gel concentration Cg was 18.82 g/L,and the ultrafiltration mass transfer coefficient k was 0.700 8.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第25期4572-4574,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(10572159)
重庆市科委科技计划项目攻关项目(2006ba5010)
高等学校学科创新引智计划(B06023)
重庆大学大型仪器设备开放基金(2074920060080)~~
