摘要
利用优化改进的Sevag试剂法对酶法提取的黑木耳粗多糖进行蛋白质的脱除。采用SephadexG-200凝胶层析对黑木耳粗多糖进行分级纯化,得到两个组分AAP1、AAP2,合并收集液,透析、浓缩、冻干得到黑木耳纯多糖,为白色粉末。采用Sephrose A-200凝胶层析、紫外光谱扫描对黑木耳纯多糖进行纯度鉴定。Sephrose A-200凝胶层析结果表明,多糖的两种组分都为单一洗脱峰;紫外光谱扫描说明多糖中不含有蛋白质、多肽及核酸物质;说明黑木耳多糖的纯度已经达到物理化学的分析标准。红外光谱扫描的结果表明,黑木耳多糖中含有多糖类物质的特征吸收峰。纸层析法对黑木耳纯多糖进行组分分析的结果表明黑木耳多糖由葡萄糖、木糖、半乳糖、甘露糖、阿拉伯糖组成,是一种杂多糖。
The protein was removed from crud auricularia auricula polysaccharide （AAP） by modified Sevag method. Two component AAP1 and AAP2 were gained by using Sephadex G-200 chromatography. The eluent was combined ,then dialysised and concentrated, freezed-drying. The pure AAP was gained. It was white powder. Then the pure AAP is identified by Sepharose A-200 chromatography, ultraviolet specrrum scanning. The result of Sepharose A-200 column chromatograpgy is that two polysaccharide components were single cleansing peak. The atlas of ultraviolet spectrum scanning indicates that there is not absorbing apex in 260nm and 280nm, and this indicates that protein, polypeptide and nucleic acid are not exist, and shows that the purification of AAP reaches the analyzing standard of physics and chemis- try. The result of IR chromatogran was that AAP1 had the characteristic absorb peak. , And the composition of monosaccharide of AAP was analyzed by chromatography, and the AAP1 is composed of glucose （Glu）, xylose（Xyl）, galactose（Gal）, mannose（Man）, arabinose（Arb）.
出处
《中国林副特产》
2011年第4期4-6,共3页
Forest By-product and Speciality in China
关键词
黑木耳
多糖
酶提取
纯化
Auricularia auricula
Polysaccharide
Enzyme i Extraction
Purification
