摘要
目的检测氨甲酰胆碱（ＣＣｈ）对人未妊娠峡部子宫平滑肌细胞（ＣＭＣ）内游离钙离子浓度（［Ｃａ２＋］ｉ）及生物合成１，４，５三磷酸肌醇（ＩＰ３）的影响，探讨Ｍ受体激动剂升高人子宫平滑肌［Ｃａ２＋］ｉ作用与影响膜肌醇磷酯代谢的关系。方法应用荧光分光光度计检测［Ｃａ２＋］ｉ水平；应用同位素放射免疫分析法检测ＩＰ３水平。结果基础状态下ＣＭＣ［Ｃａ２＋］ｉ为（１７８±２１）ｎｍｏｌ·Ｌ－１，１、１０和１００μｍｏｌ·Ｌ－１ＣＣｈ使［Ｃａ２＋］ｉ升高分别为（２４４±３１）、（３２６±３９）和（４３７±６１）ｎｍｏｌ·Ｌ－１，呈剂量依赖性；细胞外无钙时ＣＣｈ升高［Ｃａ２＋］ｉ作用被减弱；１０μｍｏｌ·Ｌ－１阿托品（Ａｔｒ）可阻断ＣＣｈ升高［Ｃａ２＋］ｉ的作用。基础状态下ＣＭＣ内ＩＰ３水平为（８１５±８６）ｎｍｏｌ·ｇ－１Ｐｒｏ。１，１０和１００μｍｏｌ·Ｌ－１的ＣＣｈ可诱导ＩＰ３水平升高，其峰值是在ＣＣｈ孵育５ｍｉｎ时，此时升高的ＩＰ３水平分别为（１０７±１５），（１３５±３１）和（１４８±２９）ｎｍｏｌ·ｇ－１Ｐｒｏ。１０μｍｏｌ·Ｌ－１Ａｔｒ可显著抑制ＣＣｈ促ＩＰ３生成作用。结论ＣＣｈ通过激动Ｍ受体使ＣＭＣ的［Ｃ?
AIM To investigate the effects of carbachol on the intracellular free calcium concentration ([Ca
2+ ] i) and the formation of inositol 1,4,5 triphosphate (IP 3) in the cervix myometrium cells
(CMC) of human and to probe into the relationship between the [Ca 2+ ] i increase and the IP
3 formation induced by muscarinic receptor agonist carbachol. METHODS Spectroflurometry
and radioimmunoassay were used to examine the [Ca 2+ ] i levels, and IP 3 levels
respectively. RESULTS In the basic condition the [Ca 2+ ] i of CMC was (178±21) nmol·L
-1 . The [Ca 2+ ] i induced by 1, 10 and 100 μmol·L -1 CCh was (244±31), (326±39)
and (437±61) nmol·L -1 respectively, which showed the dose dependent relationship. In
the calcium free medium, the [Ca 2+ ] i levels induced by CCh decreased. 10 μmol·L -1
atropine (Atr) blocked the effects of CCh on the[Ca 2+ ] i . In basic condition the IP 3 levels of
CMC was (81 5±8 6) nmol·g -1 protein. The IP 3 levels was increased by 1,10, 100 μ
mol·L -1 CCh and reached the peak at the 5 min after incubation in CCh. The peak of the IP
3 levels were (107±15), (135±31) and (148±29) nmol·g -1 proein respectively. 10 μ
mol·L -1 Atr obviously inhibited CCh induced IP 3 formation. CONCLUSION CCh dose
dependently increased the levels of [Ca 2+ ] i and the IP 3 formation in CMC by activating
muscarinic receptor. The results suggested that IP 3 induced calcium release was an
important regulatory mechanism in CCh induced [Ca 2+ ] i increase in CMC.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1999年第3期229-232,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金
关键词
氨甲酰胆碱
细胞内游离钙
浓度
子宫平滑肌
峡部
carbachol
intracellular free calcium concentration
inositol 1,4,5 triphosphate
myometrium
cervix
human
