摘要
目的:研究漆树叶对动物的皮肤刺激性与致敏性。方法:采集新鲜漆树叶(不含叶轴)冻存后粉碎,室温下将漆树叶屑用蒸馏水制成糊剂,叶和水的比例为1:1。皮肤刺激实验:新西兰纯种家兔6只,脊柱两侧备皮(3cm×3cm)后,将漆树叶糊1.0g涂敷在左侧皮肤上,覆盖4h后清洗,右侧皮肤涂0.5ml蒸馏水作为对照。清洗后1、24、48、72h观察涂敷漆树叶糊部位皮肤反应并评分。皮肤致敏实验:白化豚鼠50只,分为实验组(20只,雌雄各半)、阳性对照组(20只,雌雄各半)和阴性对照组(10只,雌雄各半)。脊柱两侧备皮(2cm×2cm)。实验开始即刻和第7、14天时,实验组背部左侧备皮处涂敷0.8g漆树叶糊,阳性对照组涂敷2.5%2,4-二硝基氯苯0.2g,阴性对照组涂蒸馏水0.4ml,均覆盖6h后清洗;实验第28天时,实验组和阴性对照组豚鼠背部左侧备皮处涂敷0.8g漆树叶糊,阳性对照组涂敷1.0%2,4-二硝基氯苯0.2g,覆盖6h后清洗。其后24、48h分别计算致敏率、判定致敏程度。结果:皮肤刺激实验:有3只家兔在涂敷漆树叶糊后皮肤出现红斑,评分平均值为0.67分(24h),刺激反应程度为轻度。皮肤致敏实验:实验组与阴性对照组所有豚鼠涂敷漆树叶糊后24、48h均未出现红斑、水肿等反应,致敏反应积分平均值为0,致敏率为0,致敏程度为弱。阳性对照组豚鼠出现红斑、水肿,48h皮肤致敏反应总积分平均值为3.65分,致敏率为100%,致敏程度为极强。结论:漆树叶对实验动物皮肤有轻度刺激性,无致敏性。
Objective： To study the skin irritation and sensitization of Toxicodendron vernicifluum （T. vernicifluum） leaf in animals. Methods： Flesh leaves （free of rachises） of T. vernicifluum were collected, frozen, and ground. Ground T. vernicifluum leaves were prepared into a paste with distilled water at room temperature; the ratio of the leaf to distilled water was 1 to 1. Skin irritation testing was conducted in 6 New Zealand white rabbits. A patch （3 cm × 3 cm） of fur of each rabbit was shaved from both sides of its back. The paste 1.0 g was applied to the bare skin on the left side of the back and maintained for 4 hours and then cleaned off. Distilled water 0.5 ml was applied to the bare skin on the right side of the back as control. Skin responses in the part applied with the paste were observed and scored 1,24,48 and 72 hours after cleaning. Skin sensitization testing was conducted in 50 albino guinea pigs. These pigs were divided into 3 group,.： the experiment group （10 males and 10 females）, the positive control group （10 males and 10 females） and the negative control g＇oup （5 males and 5 females）. A patch （2 cmx 2 cm） of fur of each pig was shaved from both sides of its back. On the beginning o[ experiment and on days 7 and 14 of experiment, the paste 0. g g, 2.5% 2,4-dinitro-chlorobenzene 0.2 g and distilled water 0.4 ml were applied to the bare skin on the left side of each pig＇s back in the experiment, positive control and negative control groups and maintained for 6 hours and then cleaned off, respectively. On days 28 of experiment, the paste 0. 8 g was applied to the bare skin on the left side of each pig＇s back in the experiment and negative control groups, 1.0% 2, 4-dinitro-chlorobenzene 0.2 g was applied to the bare skin on the left side of each pig＇s back in the positive control group and maintained for 6 hours and then cleaned. The rate and severity of sensitization were calculated and assessed 24 and 48 hours after cleaning, respectively. Results： In the
出处
《药物不良反应杂志》
2011年第3期161-164,共4页
Adverse Drug Reactions Journal
关键词
漆树叶
刺激性
致敏性
leaf of Toxicodendron vernicifluum
irritation
sensitization
