摘要
目的探索从成年大鼠心脏组织中分离、培养心脏祖细胞(CPCs)的有效方法。方法取SD大鼠心脏,经胰蛋白酶和胶原酶Ⅳ消化后,置于组织块培养基中培养,取组织块周围爬出的小、圆、亮的细胞置于心球生长培养基中培养,予以免疫组织化学鉴定。结果 2周左右可见小、圆、亮的细胞附着在组织块周围爬出的成纤维细胞层上,且其c-kit表达阳性。结论经胰蛋白酶和胶原酶Ⅳ消化的方法可以成功地对成年大鼠的CPCs进行分离和体外培养。
Objective To investigate an effective method for isolation and culture of cardiac progenitor cells（CPCs） from the heart of adult rats.Methods The hearts were obtained from SD rats and cultured in complete explants medium after digested with trypsin and collagenase Ⅳ.The small,round and bright cells migrating from tissue fragments were collected and cultured in cardiosphere-growing medium,and identified by immunohistochemistry.Results About two weeks later,some small,round and bright cells adhered to the layer of fibroblast-like cells migrating from the tissue fragment and expressed c-kit.Conclusion CPCs can be successfully isolated and cultured in vitro from adult rat by this method.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第14期1613-1615,共3页
Jiangsu Medical Journal
基金
国家自然科学基金(81070083)
关键词
心脏祖细胞
心肌再生
Cardiac progenitor cells
Myocardial regeneration
