摘要
目的观察维泰醇和紫杉醇对肿瘤细胞体外迁移能力以及血管内皮细胞管腔形成能力的影响,对它们的抗肿瘤转移能力进行比较,以证实维泰醇的抗肿瘤血管新生及抗肿瘤转移作用。方法采用SRB法,划痕试验,明胶酶谱法、ELISA、AO/EB染色法以及管腔样结构形成试验等方法观察比较维泰醇与紫杉醇的抗肿瘤转移能力。结果维泰醇对黑色素瘤细胞(B16F1)生长抑制率和致死率低于紫杉醇,维泰醇对黑色素瘤细胞迁移、MMP-2表达和对血管内皮细胞(ECV304)管腔形成的抑制能力略低于紫杉醇。结论维泰醇具有较强的抗肿瘤转移活性,虽然其抗转移能力弱于紫杉醇,但其低细胞毒特性依然有望成为新的抗肿瘤转移的药物。
To evaluate the anti-metastasis effect and the inhibition of the tube-like structure formation of human umbilical vein endothelial cells of alternol and paclitaxel.Methods SRB assay,scratch wound assay,gelatin zymography,ELISA methods,AO/EB assays and tube-like structure formation test were used to observe and compare the antitumor and antimetastasis ability. Results Alternol against melanoma cell growth inhibition rate and mortality rate were lower than paclitaxel,Alternol against melanoma cell migration,MMP-2 expression and the ability of inhibiting acknowledged formation slightly lower than paclitaxel.Results Taken together,the data suggested that alternol and paclitaxel have strong anti-metastasis activities.Alternol will be expected to become a novel,effective and low cytotoxic drug in the treatment of cancer.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2011年第8期1111-1115,共5页
Chinese Pharmacological Bulletin
基金
科技部重大新药创制科技重大专项研究资助项目(No2009ZX09103-141)
