摘要
目的探索建立适合替代气管的组织工程化气管模型。方法分离2月龄新西兰兔肋软骨细胞并传代二次后，以5×10^7／ml浓度分别种于PLGA和涤纶支架，体外环境中培养2周。将支架-软骨细胞模型植入裸鼠脊柱两侧皮下，培养4、6、8周后取出，HE染色、PAS染色、扫描电镜等观察软骨组织发育、新生毛细血管、整体组织结构层次。结果种植软骨细胞的支架经体外和体内培养，软骨细胞及基质生长分泌旺盛，支架与上皮问存在紧密的纤维连接，含有丰富的新生毛细血管。涤纶一软骨细胞模型和PLGA一软骨细胞模型可以形成完整的组织结构。结论肋软骨细胞种植于PLGA和涤纶支架材料上，经体外及体内培养构建的组织工程化气管模型符合气管替代要求。
Objective To investigate the effect of a tissue engineered trachea for replacement fabricated using three di- mensional scaffold and chondrocytes by in vitro and in vivo cuhuring. Methods Rib chondroeytes were isolated and expanded to two passages, then seeded in PLGA or Dacron scaffold at density of 5 ×10^7/ml. Cultured in vitro for two weeks, the chondrocytes-scaffold model was planted under dorsal skin between nude mice＇s spine. Histology of cartilage, neovascularization and organizational structure were observed with HE staining, PAS staining and electron nrieroscopic scan were performed after 4, 6, 8 weeks in vivo. Results Organized structure were observed in both PLGA-chondrocyte model and dacron-chondrocyte model with cartilage formation, neovaseularization and tight fibrous connective tissue between scaffold and skin after in vitro and in vivo culture. Conclusion Tissue engineered trachea fabricated using rib chondrocytes and PLGA or dacron scaffold with in vitro and in vivo culture meets the requirement of trachea replacement.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2011年第7期432-436,共5页
Chinese Journal of Thoracic and Cardiovascular Surgery
关键词
组织工程
气管
支架
软骨
组织培养
动物替代试验
Tissue engineer Trachea Stents Cartilage Tissue culture Animal testing alternatives
