摘要
目的探讨小鼠肾脏发育过程中促凋亡蛋白BAX的表达。方法选取胚龄16、18d和出生后1、3、5、7、14、21d的小鼠共54只,应用免疫组织化学、免疫荧光技术并结合体视学分析方法观察树脂切片上小鼠不同发育时期BAX的表达。结果 BAX的表达主要出现在近端小管,随着发育的不断进行,BAX的表达由皮质的近髓质区逐渐发展到中层皮质区,最后出现在浅层皮质区。BAX的面数密度从E16d到P3d逐渐升高,随后下降,在P7d又逐渐升高。结论小鼠肾脏发育过程中BAX的表达与近端小管的发育密切相关,可能影响近端小管的存活、分化和成熟。
Objective To explore the expression of BAX during mouse kidney development. Methods Fifty-four mice from embryonic day 16, 18 and postnatal day 1, 3, 5, 7, 14, 21 were chosen to investigate the expression of BAX on epoxy sections of different development phases of mouse kidney with immunohistochemistry technique and stereologieal methods. Results The immunohistochemical positive staining of BAX was mainly observed in proximal tubules. During development, the expressive position of BAX gradually moved from deep to middle cortex, and finally was located on the superficial cortex. The numerical area density of BAX gradually increased from E16d to P3d, then descend, and turned to increase again at postnatal day 7. Conclusion During the development of kidney, the expression of BAX closely relates to the growth of proximal tubules, and may impact the proximal tubules in surviving, differentiating and maturing.
出处
《解剖学报》
CAS
CSCD
北大核心
2011年第4期548-551,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30871313)
中国博士后科学基金资助项目(20070420206)
中国博士后科学基金特别资助项目(200801399)
高等学校博士学科点专项科研基金资助项目(200801590016)
辽宁省教育厅基金资助项目(2009A454)
