摘要
目的:探讨高危型HPV16 E6蛋白的表达对宫颈癌细胞株中p63α表达的影响。方法:用RT-PCR法从SiHa中扩增出带酶切位点的HPV16 E6的片段,将HPV16 E6基因片段克隆到p3×flag-CMV-Myc-24真核表达载体上,通过脂质体转染入宫颈癌细胞株后,W estern blot检测p63α的表达。结果:成功构建HPV16 E6真核表达载体,转染至宫颈癌SiHa和Caski细胞株中,p63α均低于对照组。结论:高危型HPV E6能够抑制p63α的表达,提示p63α与HPV E6的相互作用在子宫颈癌发病中发挥重要作用。
Objective：To explore the effects of expression of high risk HPV E6 protein on the expression of p63α in the cervical cancer cell lines.Methods：HPV16 E6 gene sequence were amplified from total RNA of SiHa cell lines by reverse transcription-polymerase chain reaction（RT-PCR）,then the HPV16 E6 gene sequence was inserted into the eukaryotic expression vector p3×flag-CMV-Myc-24.Western blot were used to test the expression levels of p63α after transfected into cervical cancer cell lines.Results：The HPV 16 E6 eukaryotic expression vector was successfully constructed,low expression of p63α after transfection in cervical cancer cell lines SiHa and CaSki copare to the control group.Conclusion：HR E6 protein can inhibits cervical cancer cell lines expression of p63α,p63α and HPV E6 interactions may play an important role in the pathogenesis of cervical cancer.
出处
《现代妇产科进展》
CSCD
北大核心
2011年第6期481-483,共3页
Progress in Obstetrics and Gynecology
基金
安徽省自然科学基金项目(No:090413117)
安徽省高等学校省级自然科学研究项目(No:KJ2010B375)
