摘要
目的观察不同肥胖易感性大鼠的氧化应激反应和抗氧化能力的差异。方法 40只雄性Wistar大鼠(200～220g)随机分为高脂饲料组(30只)和正常对照组(10只),高脂饲料组大鼠给予高脂饲料6周后依据体重增加量筛选出肥胖易感(OP)大鼠和肥胖抵抗(OR)大鼠各10只,OP组和OR组大鼠继续喂养高脂饲料,正常对照组大鼠给予基础饲料,13周末处死大鼠,采集样本,测定血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)以及空腹血糖(FBG)、胰岛素、甘油三酯(TG)、总胆固醇(TC)、游离脂肪酸(FFA)等指标。结果 OP组大鼠SOD含量显著低于OR组和正常对照组;OP组大鼠GSH-Px水平显著低于OR组;OP组MDA浓度显著高于正常对照组;同时OP组总能量摄入、体脂含量、血糖、血脂、血清胰岛素、胰岛素敏感指数(ISI)和游离脂肪酸水平都与OR组大鼠的差异有显著性,而OR组大鼠与正常对照组相比的差异无显著性。结论与肥胖抵抗大鼠相比,肥胖易感大鼠的氧化应激水平增高,抗氧化能力降低,血糖血脂升高并出现胰岛素抵抗。
Objective To evaluate the differences of oxidative stress level between obesity-prone rats and obesity-resistant rats.Methods Male Wistar rats were divided into control group（10 rats） fed with basal diet and high fat group（30 rats） fed with high-fat diet for 6 weeks.Ten rats in obesity-prone（OP） group and obesity-resistant（OR） group were selected from the high-fat diet group according to the gain of body weight.All rats were killed by the end of 13th week.Serum SOD,MDA,GSH-Px,blood lipids and fatty acids were detected by standard methods.Results SOD was lower in OP rats than that in OR rats and control rats.GSH-Px was lower in OP rats than that in OR rats.MDA was higher in OP rats than that in control rats.The energy intake,body fat content,FBG,serum lipids,serum insulin,ISI and free fatty acids in OP rats were significantly different from those in OR rats,but there were no differences between OR and control rats.Conclusion Compared with OR rats,the oxidative stress responses were increased and the antioxidant capacities were decreased in OP rats after 13 weeks of experiment.
出处
《卫生研究》
CAS
CSCD
北大核心
2011年第4期420-422,共3页
Journal of Hygiene Research
基金
国家863计划项目(No.2010AA023002)
国家自然科学基金(No.30771804)
关键词
肥胖
肥胖抵抗
氧化应激
抗氧化
obesity
obesity resistance
oxidative stress
antioxidant capacity
