摘要
目的:分析胃癌患者癌组织及转移淋巴结组织中胃泌素基因的表达,进一步阐明胃泌素与胃癌发生、发展的相关机制,为胃癌的防治提供实验依据。方法:收集胃癌手术患者的癌组织、癌旁组织、淋巴结组织,提取及纯化总RNA,采用Taqman实时荧光定量PCR技术测定胃泌素mRNA的表达。结果:胃泌素mRNA表达在胃癌组织较癌旁组织降低,为癌旁组织的0.063倍;淋巴组织较癌旁组织增加,为癌旁组织的35.482倍;胃泌素mRNA在胃窦癌组癌组织及淋巴组织较胃体癌组增加,分别为胃体癌组的3.074倍和9.190倍;胃泌素mR-NA在淋巴转移组的癌组织及淋巴组织较未转移组增加,分别为未转移组的59.912倍和5.895倍;胃泌素mRNA在>T2组的癌组织及淋巴组织较≤T2组增加,分别为≤T2组1.540倍和13.833倍,差异均有统计学意义(P<0.01)。结论:胃泌素在胃癌的发生、发展中有重要的作用,胃泌素与胃癌发生部位、淋巴结转移及临床分型密切相关。
Objective：To explore the relationship of expression levels of gastrin gene in matched human gastric carcinoma,lymph tissue and adjacent non-neoplastic mucosa of gastric cancer patients with development and progression of the disease.Methods：Total RNA was extracted and purified from gastric carcinoma tissues,lymph tissue and adjacent non-neoplastic mucosa of patients,and gastrin mRNA levels（mRNAL） were determined with Taqman real-time quantitative PCR.Results：The mRNAL in gastric carcinoma tissues was lower than that in adjacent non-neoplastic mucosa（with ratio of 0.063）,and was higher in metastatic lymphatic tissue than that in adjacent non-neoplastic mucosa（with ratio of 35.482）.The mRNAL was higher in carcinoma and lymphatic tissues in sinus ventriculi than those in corpus ventriculi with ratioes of 3.074 and 9.190 respectively.The mRNAL was higher in cancer and metastatic lymphatic tissues of metastasis group than those of non-metastatic group with ratioes of 9.912 and 59.912 respectively.The mRNAL was higher in cancer and metastatic lymphatic tissues of group T2 than those of group ≤T2 with ratioes of 1.540 and 13.833 respectively,and the differences were statistically significant（P0.01）.Conclusions：Gastrin has important significance in the development of gastric cancer.Its expression is highly correlated with situs,metastases and clinical manifestation of gastric cancer.
出处
《贵阳医学院学报》
CAS
2011年第3期259-262,265,共5页
Journal of Guiyang Medical College
基金
贵州省国际科技合作重点项目资助[黔科合外G字(2008)700119]
