摘要
目的研究青蒿琥酯对人胃癌细胞SGC-7901增殖、凋亡的作用及半胱氨酸天冬氨酸蛋白酶(Caspase3)蛋白表达的影响。方法将终浓度为5×104个/ml的人胃癌SGC-7901细胞置于培养板孔中,单独(空白对照)或与20、40、80μg/ml浓度的青蒿琥酯培养,然后加入四唑氮蓝(MTT)溶液共同培育。采用MTT法检测青蒿琥酯对人胃癌SGC-7901细胞生长的影响。SGC-7901细胞在培养板中培养后,应用倒置显微镜、DNA琼脂糖凝胶电泳梯状条带法观察细胞的凋亡情况。Caspase3蛋白试剂盒检测Caspase3酶活性的变化情况。结果青蒿琥酯对人胃癌细胞SGC-7901细胞体外增殖有明显的抑制作用,其抑制率随时间延长(6、12、24h)和药物浓度增加(20、40、80μg/ml)而增强(21.89%、32.20%、47.85%,P<0.05);青蒿琥酯处理24h后,细胞形态学观察到细胞凋亡坏死;琼脂糖电泳观察到明显DNA片段化;Caspase3的酶活性伴随着药物浓度的增加(0、20、40、80μg/ml)而增高〔(2.57±0.32)、(5.58±0.49)、(6.87±0.73)、(10.21±0.57)U/ml,P<0.05〕。结论青蒿琥酯能够抑制人胃癌细胞SGC-7901的增殖,促进其凋亡。Caspase3蛋白的高表达在促进SGC-7901细胞凋亡中起着重要作用。
Objective To study the effects of artesunate on proliferation and apoptosis of human gastric cancer SGC-7901 cell and expression of Caspase 3 protein.Methods 5×104 cell/ml gastric cancer SGC-7901 cells were placed in the wells of culture plate and incubated alone（blank control） or with 20,40,80μg /ml artesunate,and tetrazolium blue nitrogen （MTT） solution was added to incubate together.MTT method was used to detect the influence of artesunate on the growth of SGC-7901 cells;cell apoptosis was observed by inverted microscope and DNA ladder of agarose gel electrophoresis after culture in culture plate;changes of Caspase 3 activity were detected by caspase 3 kit.Results The proliferation of human gastric cancer SGC-7901 cells were significantly inhibited by artesunate in vitro,and the inhibition rates enhanced in proportion to increase of time（6,12,24 h） and drug concentration（21.89%,32.20%,47.85%,P0.05）.The changes of cytomorphology showed that cell apoptosis and necrosis was obvious,and DNA fragmentation was markedly observed by agarose gel electrophoresis.The activity of Caspase 3 increased〔（2.57±0.32）,（5.58±0.49）,（6.87±0.73）,（10.21±0.57） U/ml）〕 in proportion to increase of artesunate concentration （0,20,40,80 μg /ml） （P0.05）.Conclusions Artesunate can inhibit proliferation of human gastric cancer cell SGC-7901 and promote their apoptosis.The hyper-expression of Caspase 3 protein might play an important role in promoting apoptosis of human gastric cancer SGC-7901 cells.
出处
《中国临床研究》
CAS
2011年第7期556-558,共3页
Chinese Journal of Clinical Research
