摘要
ACOS5是控制拟南芥雄性育的关键基因之一,它也是编码花粉素合成必需的酶.利用氨基酸同源序列分析,在数据库中检索与拟南芥ACOS5同源性较高的植物同源蛋白.将检索出的水稻中同源氨基酸序列与ACOS5进行比对,两者一致性为64%.并且通过同源蛋白系统发生分析,两者在进化上关系也较为密切.根据水稻中同源蛋白基因组序列设计引物,进行低温处理下该基因在小花发育各时期的表达量分析.半定量RT-PCR显示,在水稻小孢子母细胞形成期其表达量较低,而当小孢子开始减数分裂时,表达量急剧增高.在这两个时期,低温(20℃)会诱导OsACOSmRNA积累增加;但当处于小孢子形成阶段时,低温下积累量却呈现相反的降低趋势.这说明环境温度显著影响水稻中ACOS5同源基因的转录表达,而这种影响与小花发育时期有关,提示水稻ACOS基因可能参与环境温度调控水稻雄性育性的过程.
ACOS5 encodes and enzyme required for sporopdlen monomer biosynthesis and is one of the crucial genes for normal male fertility in Arabidopsis.Blast analysis exhibited that several proteins from distinct plant species showed high homologoue to ACOS5 and there were 64% identities at amino acid level between ACOS5 and its rice homologue（OsACOS）.The phylogenetic analysis further indicated their tight relationship in evolution.RT-PCR analysis of OsACOS in several stages floret treated by low and high temperature respectively was performed using the primers based on the rice homologous sequence.The result demonstrated that the expression level of OsACOS was low at the microsporocyte formation stage,whereas it was increased dramatically from the meiosis stage.During these two early stages,significantly higher level of OsACOS mRNA abundance was induced at the lower temperature（20 ℃） compared with that at the higher temperature.However,this trend was reversed at the formation of microspores stage.Therefore,the ambient temperature significantly imposes the transcription expression of OsACOS and the effect was developmental stage-dependent.It suggested that OsACOS might be involved in the regulatory pathway through which the ambient temperature controls the male fertility of rice.
出处
《上海师范大学学报（自然科学版）》
2011年第3期284-288,共5页
Journal of Shanghai Normal University(Natural Sciences)
基金
上海市高校选拔优秀青年教师科研专项基金
上海市教委浦江人才计划(08PJ1405500)
上海市教委2008年创新项目
上海大学科技创新基金
