摘要
首次建立了N-溴代丁二酰亚胺(NBS)-曙红Y(EY)化学发光体系用于检测盐酸吗啉胍,在本化学发光体系中NBS为氧化剂、EY为能量受体。在碱性条件下,NBS氧化盐酸吗啉胍产生稳定的化学发光,向体系中加入十六烷基三甲基溴化胺(CTMAB)后,化学发光显著增强。研究了NBS浓度、EY浓度、CTMAB浓度、NaOH浓度、阀池管长和泵速等因素对化学发光强度的影响,初步探讨了此化学发光可能的机理。结果显示:在最佳实验条件下,盐酸吗啉胍的检测线性范围为4.7×10-8～2.0×10-5 g.mL-1,线性回归方程为I=19.513C(×10-7 g.mL-1)+26.118,R2=0.9995,方法检出限(DL=3σn-1/S)为1.6×10-8 g.mL-1。对盐酸吗啉胍片剂(标示量为0.1 g.片-1)中盐酸吗啉胍定量分析,结果平均为0.0977 g.片-1,相对标准偏差为2.0%,加标回收率为93.5%～102.6%,结果令人满意。
A novel N-bromosuccinimide（NBS）-Eosin Y（EY） chemiluminescence（CL） system for the determination of moroxydine hydrochloride coupled with flow injection analysis was proposed.It was found that chemiluminescence was taken by NBS oxidation moroxydine hydrochlorid with EY as energy transfer in alkaline.This system was enhanced by hexadecyl trimethyl ammonium bromide（CTMAB）.The effects of various factors（NBS concentration,EY concentration,CTMAB concentration,NaOH concentration,peristaltic pump pipe range and flow rates） on the CL system were investigated.The possible mechanism was also studied preliminarily.The line arrange of determination was 4.7×10-8～2.0×10-5 g·mL-1 and the equation of linear regression was I=19.513C（×10-7 g·mL-1）＋26.118,R2=0.9995.The detection limit（DL=3σn-1/S） was 1.6×10-8 g·mL-1.Using this CL system,the detection results of moroxydine hydrochloride in tablets（0.0977 g/tablet） compared well with the labeled amount（0.1 g/tablet）,the value of RSD was found to be 2.0%,and the recovery was satisfactory with 93.5%～102.6%.
出处
《广州化工》
CAS
2011年第13期110-112,140,共4页
GuangZhou Chemical Industry
