摘要
目的探讨组织因子途径抑制物(TFPI)基因多态性与肺血栓栓塞症(PTE)的关系。方法选择PTE患者35例,健康对照组34名。采用聚合酶链反应(PCR/RT-PCR)检测PTE组和健康对照组TFPIC-399T、T-287C基因型及等位基因频率,结果经测序证实。结果 PTE组C-399T纯合突变型TT、杂合子突变型CT和正常纯合子CC与健康对照组之间差异无统计学意义(P>0.05);T和C等位基因型频率与健康对照组比较差异无统计学意义。PTE组T287-C杂合子突变型CT、纯合突变CC和正常纯合子CC与健康对照组比较差异无统计学意义(P>0.05);T和C等位基因频率分布与对照组比较,差异也无统计学意义(P>0.05)。结论 TFPI基因C-399T、T287-C多态性可能与PTE无相关性。
Objective To explore the correlation between the gene polymorphism of tissue factor pathway inhibitor（TFPI） and pulmonary thromboemlism（PTE）.Methods Thirty-five PTE patients were recuited,with 34 healthy people assigned as normal controls.TFPI C-399T and T-287C genotypes and the allele frequencies were detected in the PTE group and the normal control group by RT-PCR.The results were then verified by sequencing.Results There was no significant difference in C-399T homozygous mutant TT,heterozygote-mutant CT and normal homozygote CC between PTE and normal control groups（P〉0.05）.No statistical difference was found in T and C allele frequencies between these two groups.Compared with the normal control group,PTE group showed no statistaical difference in T287-C heterozygote-mutant CT,homozygous mutant CC and normal homozygote CC（P〉0.05）.In addition,no difference was found in distribution of T and C allele frequencies in the two groups（P〉0.05）.Conclusion There might be no correlation between gene polymorphism of TFPI genetypes（C-399T plus T287-C） and PTE.
出处
《中国药物与临床》
CAS
2011年第7期758-762,共5页
Chinese Remedies & Clinics
基金
山西省自然科学基金(2010011050-6)
关键词
多态性
单核苷酸
肺栓塞
组织因子途径抑制物
Polymorphism
single nucleotide
Pulmonary embolism
Tissue factor pathway inhibitor
