摘要
目的探讨慢性乙型肝炎患者乙型肝炎e抗原(HBeAg)阳性与阴性血清乙肝病毒脱氧核糖核酸(HBVDNA)水平与肝组织炎症分级的相关性。方法将121例慢性乙型肝炎患者分为HBeAg阳性组(62例)及阴性组(59例),乙型肝炎病毒(HBV)血清标志物用化学发光法检测;丙氨酸氨基转移酶(ALT)用全自动生化分析仪检测;血清HBV DNA定量检测采用荧光定量聚合酶链反应法;肝组织行常规病理染色判断炎症活动度。结果 HBeAg阴性组和阳性组平均年龄及病程分别为(36.95±8.5)岁和(33.23±10.42)岁(,118.79±72.29)个月和(82.45±52.24)个月(两组比较均P<0.05);HBeAg阳性组和阴性组血清HBV DNA定量及ALT水平分别为(6.43±1.18)IU.mL-1 vs(4.16±2.48)IU.mL-1(、222.54±272.68)IU.L-1 vs(134.88±120.85)IU.L-1(两组比较均P<0.05);HBeAg阳性组血清HBV DNA定量与肝组织炎症分级相关性检验rs=-0.095,P>0.05;HBeAg阴性组血清HBV DNA定量与肝组织炎症分级相关性检验rs=0.261,P<0.05。结论 HBeAg阳性与阴性的慢性乙型肝炎的的临床表现及病理学特点有所不同,慢性乙型肝炎的病变程度应综合一般临床资料、血清HBV DNA水平及肝穿刺病理来共同评判。
Objective To investigate the relationship between serum HBV DNA levels and liver inflammation grade（G） in chronic hepatitis B patients with HBeAg-positive and HBeAg-negative.Methods 121 patients with chronic hepatitis B were divided into HBeAg-positive group（62 cases） and HBeAg-negative（59 cases）.The serum hepatitis B virus（HBV） markers were detected by chemiluminescence.The alanine aminotransferase（ALT） were detected by automatic biochemical analyzer.The serum HBV DNA quantitation were detected by fluorescence quantitative PCR.The liver tissue was stained to evaluate the degree of inflammation.Results The average age and the course of disease in HBeAg-negative group were higher than those in HBeAg-positive group[（36.95 ±8.5）years vs（33.23±10.42）years,（118.79±72.29）months vs（82.45±52.24）months（P0.05）].The serum HBV DNA quantitation and ALT levels in HBeAg-positive group were higher than those in HBeAg-negative group[（6.43±1.18）IU·mL-1 vs（4.16±2.48）IU·mL-1、（222.54±272.68） IU·L-1 vs（134.88±120.85） IU·L-1（P0.05）].The serum HBV DNA quantitation of HBeAg-positive group showed no correlation with liver inflammation degree（rs=-0.095,P0.05）.The serum HBV DNA quantitation of HBeAg negative showed a positive correlation with liver inflammation degree（rs=0.261,P0.05）.Conclusion The chronic hepatitis B patients with HBeAg-positive and HBeAg-negative had different clinical manifestations and hepatic pathological features.It is necessary for evaluating the degree of disease in chronic hepatitis B patients to consider general clinical data,serum HBV DNA levels and hepatic pathological together.
出处
《宁夏医科大学学报》
2011年第6期525-527,共3页
Journal of Ningxia Medical University
基金
宁夏回族自治区自然科学基金(NZ10148)
