摘要
目的检测AnnexinA2和P19在原发性肝癌中的表达并探讨其在肝癌发生发展及转移中的作用。方法原发性肝癌62例,分别取癌组织及癌旁肝组织,采用IHC、RT-PCR及Western blot法分别检测AnnexinA2和P19 mRNA及蛋白的表达。结果肝癌组织中AnnexinA2 mRNA及蛋白表达量均明显高于癌旁肝组织(P<0.05),肝癌组织中P19 mRNA及蛋白表达量均明显低于癌旁肝组织(P<0.05);AnnexinA2、P19阳性表达与肝癌患者的性别、年龄和肿瘤大小均无关(P>0.05),而与乙肝表面抗原、组织分级和脉管内有无癌栓均有关(P<0.05)。结论联合检测AnnexinA2、P19 mRNA及蛋白在原发性肝癌中的表达水平,对判断其发生和转移有重要价值,可为临床治疗和判断预后提供依据。
Objective To detect the expression of AnnexinA2 and P19 in patients with primary hepatic carcinoma and to explore the effect of their expression in occurrence,development and metastasis of primary hepatic carcinoma.Methods RT-PCR、Western blot and IHC techniques were used to detect the expression of AnnexinA2 and P19 in patients with primary hepatic carcinoma.Results AnnexinA2 and P19 expression in primary hepatic carcinoma tissues were significantly different from those in the adjacent liver tissues.The expression of AnnexinA2 in the primary hepatic carcinoma tissues was significantly higher than that in the adjacent liver tissues（P0.05）.The expression of P19 in the primary hepatic carcinoma tissue was significantly lower than that in the adjacent liver tissues（P0.05）.Conclusion AnnexinA2 and P19 expression has important value for prediction of occurrence and metastasis.They maybe supply evidence for clinical therapy and judgement of prognosis.
出处
《宁夏医科大学学报》
2011年第6期511-514,F0002,共5页
Journal of Ningxia Medical University
基金
国家自然科学基金(81060044)
