摘要
首次采用高速逆流色谱法对经自制聚酰胺初步分离的表没食子儿茶素-3-(3″-O-甲基)没食子酸酯(EGCG3″Me)样品中的EGCG3″Me单体进行分离纯化。结果表明,选择水-甲醇-乙酸乙酯-正己烷(体积比5∶2∶9∶1)为高速逆流色谱分离的两相溶剂系统,上相为固定相,下相为流动相,在主机转速700 r/min、流速2.0 mL/min、检测波长254 nm的条件下,可对EGCG3″Me实现良好分离。经高效液相色谱检测,分离纯化后的EGCG3″Me纯度可达90%以上,产物的收率为76.53%。
A method for the isolation and purification of（-）-epigallocatechin-3-（3″-O-methyl）gallate（EGCG3″Me） from crude extract sample was firstly developed by high speed countercurrent chromatography（HSCCC）.The results showed that HSCCC had a two-phase solvent system composing of water-methanol-ethyl acetate-hexane（5 ∶2 ∶9 ∶1,by volume） on a preparative scale.The upper phase was used as the stationary phase,and the lower phase was used as the mobile phase.The revolution speed was 700 r/min,the flow rate was 2.0 mL/min and the detection wavelength was set at 254 nm.Under the optimized conditions,EGCG3″Me can be well isolated.The purity of EGCG3″Me detected by high performance liquid chromatography was more than 90%,and the yield of EGCG3″Me by HSCCC was 76.53%.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第7期813-816,共4页
Journal of Instrumental Analysis
基金
重庆市科委项目资助(CSTC.2007AC1044)
关键词
EGCG3″Me
高速逆流色谱
分离纯化
高效液相色谱
EGCG3″Me
high speed countercurrent chromatography
isolation and purification
high performance liquid chromatography
