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血链球菌和牙龈卟啉单胞菌在SPF大鼠口腔中的相互作用 被引量:2

Investigation on Interaction Between Streptococcus sanguis and \;Porphyromonas gingivalis in Specific Pathogen free Rats
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摘要 目的:研究从SPF大鼠口腔中分离的内源性血链球菌是否可以阻止或减少牙周可疑致病菌牙龈卟啉单胞菌P.g381在口腔的生长和定植。方法:选用10只SPF大鼠分作2组,强力霉素口腔清扫7d以后分别接种内源性血链球菌和P.g381,二者均获稳定定植。接着选用30只SPF大鼠经强力霉素口腔清扫后随机分作A~F6组,其中A、B两组先接种P.g3815d,C、E两组先接种血链球菌5d;然后A组再接种血链球菌5d,C、D两组接种P.g3815d,F组作为阴性对照。接种完成后分别于12h、24h、36h、7d和14d采集龈下菌斑标本检测内源性血链球菌和P.g381定植水平。结果:先定植和重复感染的内源性血链球菌在较短的时间内(36h内)可以减少P.g381的定植水平。这并非是由于24h或36h以后血链球菌水平下降所致,因为整个实验中血链球菌始终保持稳定的定植水平。结论:血链球菌不仅要具备定植于口腔的能力,更重要的是能在活体有效地发挥其拮抗作用。 Objective: To examine whether endogenous S.sanguis could prevent, or reduce the colonization of the virulent P.gingivalis strain. Methods: First, 10 specific pathogen free Wistar rats were divided into 2 groups. Doxycycline was administered in the drinking water for 7 days. Successful implantation of the endogenous strain of S.sanguis, isolated from one of the rats before doxycycline administration, and P.gingivalis 381 within 14 days of observation were demonstrated in the rats of each group respectively. Then, 30 SPF rats were divided into 6 groups. Doxycycline was administered in the drinking water for 7 days to all the rats. Afterwards, the rats in group A and B were inoculated orally once a day for 5 days with P.gingivalis, the rats in group C and E were inoculated orally once a day for 5 days with S.sanguis. Then, the rats in group A were inoculated for 5 days with S.sanguis, and rats in group C and D were inoculated for 5 days with P.gingivalis. The rats in group F served as negative control. After inoculation, the levels of S.sanguis and P.gingivalis in the mouths of the rats were determined after 12, 24, 36 hours, 7 days and 14 days. Results: Both pre colonization of S.sanguis and superinfection with S.sanguis did reduce the level of P.gingivalis in experimental rats. However, the reduction only maintained quite short time, about 36 hours. It was not caused by the decreased level of S.sanguis after 36 hours because the level of S.sanguis kept stable during the observation period of 14 days. Conclusion: That S.sanguis function as the effector strain requires the successful implantation of S.sanguis as well as S.sanguis producing antagonitic action efficiently in vivo.\;
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 1999年第4期310-313,共4页 West China Journal of Stomatology
基金 国家教委博士点基金
关键词 血链球菌 牙龈 卟啉单胞菌 SPF大鼠 牙周病 Streptococcus sanguis\ \ Porphyromonas gingivalis\ \ replacement therapy\ \ SPF rats
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