摘要
[目的]研究当归补血汤对肉鸡免疫脏器指数和红细胞免疫功能的影响。[方法]选取100只1日龄健康三黄鸡,随机分成5组。7日龄时,通过滴鼻的方法接种鸡新城疫疫苗,并分别在试验Ⅰ、Ⅱ和Ⅲ组鸡的饮水中添加2%、5%和10%的当归补血汤,Ⅳ组在饮水中添加2%的黄芪多糖,Ⅴ组为空白对照组。分别于14、21和28日龄进行颈静脉采血和剖检,测定各免疫器官脏器指数、红细胞E-C3bRR的花环率和红细胞免疫复合物E-ICR的花环率。试验期28 d。[结果]当归补血汤对胸腺免疫脏器指数无显著影响,能显著提高脾脏和法氏囊脏器指数。在外周和体液免疫功能方面,以添加10%的当归补血汤效果最佳,其对机体的外周免疫和体液免疫效果均优于2%的黄芪多糖。[结论]当归补血汤对鸡的免疫脏器指数及红细胞免疫功能有显著的影响作用,可作为免疫增强剂加以应用。
Abstract [ Objective ] To study the effects of Dangguibuxue decoction＇on immune organ and erythrocyte immune in chickens. [ Method ] One hundred 1 -day -old healthy Sanhuang chickens were randomly divided into five groups, 20 in each group. They were imnmnized with Newcastle disease vaccine via intranasal inoculation at 7 days old. The chickens in group I, Ⅱ and Ⅲ drank water added the Danggguibuxue decoction al proportion of 2%, 5% and 10% （ V/V）, respectively. The chickens in the group IV drank water added Astragalus polysaccharide at propoltion of 2% （V/V）. And those in the group V drank water without addition of any drug. Blood was collected via jugular vein at 14, 21 and 28 days old, respectively, and then they were dissected. The immune organ indexes, erythrocyte C^3b receptor rosette rate （E-C3b RR） and erythrocyte im- mune complex rosette rate （E-ICR） were measured. [ Result] The Dangguibuxue decoction had no significant effect on thymus index but signifi- cantly increased spleen index and bursa index. The best peripheral and humoral immunity was observed in the chickens drinking water added the Dangguibuxue decoction at proportion of 10% （ V/V）, and its immune enhancement was better than that of Astragalus polysaccbaride added at proportion of 2% （V/V）. [ Conclusion] Dangguibuxue decoction can increase immune organ indexes and enhance erythrocyte immune functions; thus, it can be used as inmmnopotentiator.
出处
《安徽农业科学》
CAS
北大核心
2011年第18期11100-11102,共3页
Journal of Anhui Agricultural Sciences
基金
教育部<长江学者和创新团队发展计划>创新团队项目(IR-TO848)
2010年本科科研兴趣项目
关键词
当归补血汤
鸡
免疫器官指数
红细胞免疫
Dangguibuxue decoction
Chickens
Immune organ indexes
Elythrocyte immune
